β-Cell-specific inactivation of the mouseIpf1/Pdx1 gene results in loss of the β-cell phenotype and maturity onset diabetes
Open Access
- 15 June 1998
- journal article
- Published by Cold Spring Harbor Laboratory in Genes & Development
- Vol. 12 (12), 1763-1768
- https://doi.org/10.1101/gad.12.12.1763
Abstract
To study the late β-cell-specific function of the homeodomain protein IPF1/PDX1 we have generated mice in which theIpf1/Pdx1 gene has been disrupted specifically in β cells. These mice develop diabetes with age, and we show that IPF1/PDX1 is required for maintaining the β cell identity by positively regulating insulin and islet amyloid polypeptide expression and by repressing glucagon expression. We also provide evidence that IPF1/PDX1 regulates the expression of Glut2 in a dosage-dependent manner suggesting that lowered IPF1/PDX1 activity may contribute to the development of type II diabetes by causing impaired expression of both Glut2 and insulin.Keywords
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