Easy labeling of proliferative phase and sporogonic phase of microsporidia Nosema bombycis in host cells
Open Access
- 22 June 2017
- journal article
- research article
- Published by Public Library of Science (PLoS) in PLOS ONE
- Vol. 12 (6), e0179618
- https://doi.org/10.1371/journal.pone.0179618
Abstract
Microsporidia are eukaryotic, unicellular parasites that have been studied for more than 150 years. These organisms are extraordinary in their ability to invade a wide range of hosts including vertebrates and invertebrates, such as human and commercially important animals. A lack of appropriate labeling methods has limited the research of the cell cycle and protein locations in intracellular stages. In this report, an easy fluorescent labeling method has been developed to mark the proliferative and sporogonic phases of microsporidia Nosema bombycis in host cells. Based on the presence of chitin, Calcofluor White M2R was used to label the sporogonic phase, while β-tubulin antibody coupled with fluorescence secondary antibody were used to label the proliferative phase by immunofluorescence. This method is simple, efficient and can be used on both infected cells and tissue slices, providing a great potential application in microsporidia research.Keywords
Funding Information
- National Natural Science Foundation of China (31602012)
- National Natural Science Foundation of China (31470250)
- National Natural Science Foundation of China (31402138)
- China Postdoctoral Science Foundation (2015M570763)
- Fundamental Research Funds for the Central Universities (XDJK2015C004)
- Fundamental Research Funds for the Central Universities (XDJK2015A010)
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