Mouse embryos with paternal duplication of an imprinted chromosome 7 region die at midgestation and lack placental spongiotrophoblast
Open Access
- 1 January 1996
- journal article
- Published by The Company of Biologists in Development
- Vol. 122 (1), 265-270
- https://doi.org/10.1242/dev.122.1.265
Abstract
Imprinted genomic regions have been defined by the production of mice with uniparental inheritance or duplication of homologous chromosome regions. With most of the genome investigated, paternal duplication of only distal chromosomes 7 and 12 results in the lack of offspring, and prenatal lethality is presumed. Aberrant expression of imprinted genes in these two autosomal regions is therefore strongly implicated in the periimplantation lethality of androgenetic embryos. We report that mouse embryos with paternal duplication of distal chromosome 7 (PatDup.d7) die at midgestation and lack placental spongiotrophoblast. Thus, the much earlier death of androgenones must involve paternal duplication of other autosomal regions, acting independently of or synergistically with PatDup.d7. The phenotype observed is similar, if not identical to, that resulting from mutation of the imprinted distal chromosome 7 gene, Mash2, which in normal midgestation embryos exhibits spongiotrophoblast-specific maternally active/paternally inactive (m+/p−) allelic expression. Thus, the simplest explanation for the PatDup.d7 phenotype is p−/p− expression of this gene. We also confirm that PatDup.d7 embryos lack H19 RNA and possess excess Igf2 RNA as might be expected from the parental-specific activities of these genes in normal embryos.Keywords
This publication has 33 references indexed in Scilit:
- Parental imprinting of autosomal mammalian genesCurrent Opinion in Genetics & Development, 1994
- A candidate mouse model for Prader–Willi syndrome which shows an absence of Snrpn expressionNature Genetics, 1992
- Physical linkage of two mammalian imprinted genes, H19 and insulin–like growth factor 2Nature Genetics, 1992
- Embryological and molecular investigations of parental imprinting on mouse chromosome 7Nature, 1991
- Role of paternal and maternal genomes in mouse developmentNature, 1984
- Inviability of parthenogenones is determined by pronuclei, not egg cytoplasmNature, 1984
- Completion of mouse embryogenesis requires both the maternal and paternal genomesCell, 1984
- Factors affecting the observed number of young resulting from adjacent-2 disjunction in mice carrying a translocationGenetics Research, 1977
- Further observations on the hairpin-tail (Thp) mutation in the mouseGenetics Research, 1974
- Do the H-2 and T-Loci of the Mouse have a Function in the Haploid Phase of Sperm ?Nature, 1972