Deep sequencing uncovers commonality in small RNA profiles between transgene-induced and naturally occurring RNA silencing of chalcone synthase-A gene in petunia
Open Access
- 30 January 2013
- journal article
- Published by Springer Science and Business Media LLC in BMC Genomics
- Vol. 14 (1), 63
- https://doi.org/10.1186/1471-2164-14-63
Abstract
Introduction of a transgene that transcribes RNA homologous to an endogenous gene in the plant genome can induce silencing of both genes, a phenomenon termed cosuppression. Cosuppression was first discovered in transgenic petunia plants transformed with the CHS-A gene encoding chalcone synthase, in which nonpigmented sectors in flowers or completely white flowers are produced. Some of the flower-color patterns observed in transgenic petunias having CHS-A cosuppression resemble those in existing nontransgenic varieties. Although the mechanism by which white sectors are generated in nontransgenic petunia is known to be due to RNA silencing of the CHS-A gene as in cosuppression, whether the same trigger(s) and/or pattern of RNA degradation are involved in these phenomena has not been known. Here, we addressed this question using deep-sequencing and bioinformatic analyses of small RNAs.Keywords
This publication has 55 references indexed in Scilit:
- Plant secondary siRNA production determined by microRNA-duplex structureProceedings of the National Academy of Sciences of the United States of America, 2012
- Suppressive mechanism of seed coat pigmentation in yellow soybeanBreeding Science, 2012
- 22-nucleotide RNAs trigger secondary siRNA biogenesis in plantsProceedings of the National Academy of Sciences of the United States of America, 2010
- Unique functionality of 22-nt miRNAs in triggering RDR6-dependent siRNA biogenesis from target transcripts in ArabidopsisNature Structural & Molecular Biology, 2010
- Endogenous, Tissue-Specific Short Interfering RNAs Silence the Chalcone Synthase Gene Family inGlycine maxSeed CoatsPlant Cell, 2009
- Distinct extremely abundant siRNAs associated with cosuppression in petuniaRNA, 2009
- The Spliceosome: Design Principles of a Dynamic RNP MachineCell, 2009
- Genome-Wide Analysis of the RNA-DEPENDENT RNA POLYMERASE6/DICER-LIKE4 Pathway inArabidopsisReveals Dependency on miRNA- and tasiRNA-Directed TargetingPlant Cell, 2007
- RNA interference‐inducing hairpin RNAs in plants act through the viral defence pathwayEMBO Reports, 2006
- RNA silencing in plantsNature, 2004