Expression of a Cloned Rat Brain Potassium Channel in Xenopus Oocytes
- 14 April 1989
- journal article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 244 (4901), 221-224
- https://doi.org/10.1126/science.2539643
Abstract
Potassium channels are ubiquitous membrane proteins with essential roles in nervous tissue, but little is known about the relation between their function and their molecular structure. A complementary DNA library was made from rat hippocampus, and a complementary DNA clone (RBK-1) was isolated. The predicted sequence of the 495-amino acid protein is homologous to potassium channel proteins encoded by the Shaker locus of Drosophila and differs by only three amino acids from the expected product of a mouse clone MBK-1. Messenger RNA transcribed from RBK-1 in vitro directed the expression of potassium channels when it was injected into Xenopus oocytes. The potassium current through the expressed channels resembles both the transient (or A) and the delayed rectifier currents reported in mammalian neurons and is sensitive to both 4-aminopyridine and tetraethylammonium.Keywords
This publication has 25 references indexed in Scilit:
- At least two mRNA species contribute to the properties of rat brain A-type potassium channel expressed in xenopus oocytesNeuron, 1988
- Four cDNA clones from the Shaker locus of Drosophila induce kinetically distinct A-type potassium currents in Xenopus oocytesNeuron, 1988
- Expression of functional potassium channels from Shaker cDNA in Xenopus oocytesNature, 1988
- Cloning of Genomic and Complementary DNA from Shaker , a Putative Potassium Channel Gene from DrosophilaScience, 1987
- Sequence of a Probable Potassium Channel Component Encoded at Shaker Locus of DrosophilaScience, 1987
- Two Mammalian Genes Transcribed from Opposite Strands of the Same DNA LocusScience, 1987
- The Use ofXenopusOocytes for the Study of Ion ChannelCritical Reviews in Biochemistry, 1987
- Primary structure of Electrophorus electricus sodium channel deduced from cDNA sequenceNature, 1984
- Conduction and selectivity in potassium channelsThe Journal of Membrane Biology, 1983
- Isolation of biologically active ribonucleic acid from sources enriched in ribonucleaseBiochemistry, 1979