Cooling and Freezing of Boar Spermatozoa: Supplementation of the Freezing Media With Reduced Glutathione Preserves Sperm Function
- 6 May 2005
- journal article
- research article
- Published by Wiley in Journal of Andrology
- Vol. 26 (3), 396-404
- https://doi.org/10.2164/jandrol.04155
Abstract
In this study, we evaluated the effects of glutathione (L-gamma-glutamyl-L-cysteinylglycine; GSH) supplementation of the freezing extender on semen parameters during the cooling (2 hours at 5 degrees C) and freezing phases of the cryopreservation process to compensate for the decrease in GSH content observed during sperm freezing. To fully address these questions, we incorporated a new set of functional sperm tests. These included tests of mitochondrial function, inducibility of the acrosome reaction, in vitro penetration (IVP) of oocytes, changes in sulfhydryl group content in membrane proteins, and capacitation status. The main findings emerging from this study were that the addition of GSH to the freezing media resulted in 1) an improvement in percent motility (%MOT) and motion parameters of thawed spermatozoa, as measured by both microscopic analysis and computer-assisted semen analysis (CASA); 2) a higher number of total viable spermatozoa; 3) a higher number of noncapacitated viable spermatozoa; and 4) a decrease in the number of spermatozoa with changes in the sulfhydryl groups in membrane proteins. This protective effect on sperm function was more pronounced with 1 mM of GSH than with 5 mM of GSH.Keywords
This publication has 34 references indexed in Scilit:
- Sperm factors related to in vitro and in vivo porcine fertilityTheriogenology, 2005
- Decrease in glutathione content in boar sperm after cryopreservation: Effect of the addition of reduced glutathione to the freezing and thawing extendersTheriogenology, 2004
- Laboratory Semen Assessment and Prediction of Fertility: still Utopia?*Reproduction in Domestic Animals, 2003
- Cryopreservation alters membrane sulfhydryl status of bull spermatozoa: Protection by oxidized glutathioneMolecular Reproduction and Development, 2001
- Porcine sperm fertilizing potential in relationship to sperm functional capacitiesAnimal Reproduction Science, 1996
- Glutathione as a treatment for male infertilityReviews of Reproduction, 1996
- Recent developments and concepts in the cryopreservation of spermatozoa and the assessment of their post-thawing functionReproduction, Fertility and Development, 1995
- Analysis of Sperm Cell Viability, Acrosomal Integrity, and Mitochondrial Function Using Flow Cytometry1Biology of Reproduction, 1990
- Role of glutathione peroxidase in protecting mammalian spermatozoa from loss of motility caused by spontaneous lipid peroxidationGamete Research, 1989
- An Investigation of the Effectiveness of Certain Antioxidants in Preserving the Motility of Reactivated Bull Sperm Models1Biology of Reproduction, 1988