Zinc Efficiency Is Correlated with Enhanced Expression and Activity of Zinc-Requiring Enzymes in Wheat
Open Access
- 1 February 2003
- journal article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 131 (2), 595-602
- https://doi.org/10.1104/pp.011825
Abstract
Zinc (Zn) is an essential micronutrient for plants. The ability of plants to maintain significant yields under low Zn is termed Zn efficiency (ZE) and its genetic and mechanistic basis is still not well understood. Previously, we showed that root Zn uptake did not play a role in ZE. In the current study, Zn-efficient and -inefficient wheat (Triticum aestivum) genotypes were grown for 13 d in chelate buffer nutrient solutions at low (0.1 pm), sufficient (150 pm), and high (1 μm) Zn2+ activities and analyzed for root-to-shoot translocation of Zn, subcellular leaf Zn distribution, and activity and expression of the Zn-requiring enzymes in leaves. No correlation between ZE and Zn translocation to the shoot was found. Furthermore, total and water-soluble concentrations of leaf Zn were not associated with ZE, and no differences in subcellular Zn compartmentation were found between Zn-efficient and -inefficient genotypes. However, the expression and activity of the Zn-requiring enzymes copper (Cu)/Zn superoxide dismutase (SOD) and carbonic anhydrase did correlate with differences in ZE. Northern analysis suggested that Cu/ZnSOD gene expression was up-regulated in the Zn-efficient genotype, Kirgiz, but not in inefficient BDME. Under Zn deficiency stress, the very Zn-efficient genotype Kirgiz and moderately Zn-efficient Dagdas exhibited an increased activity of Cu/ZnSOD and carbonic anhydrase when compared with Zn-inefficient BDME. These results suggest that Zn-efficient genotypes may be able to maintain the functioning of Zn-requiring enzymes under low Zn conditions; thus, biochemical Zn utilization may be an important component of ZE in wheat.Keywords
This publication has 24 references indexed in Scilit:
- High- and Low-Affinity Zinc Transport Systems and Their Possible Role in Zinc Efficiency in Bread WheatPlant Physiology, 2001
- Tansley Review No. 111New Phytologist, 2000
- Carbonic Anhydrase Activity and CO2-Transfer Resistance in Zn-Deficient Rice LeavesPlant Physiology, 1998
- Studies on the distribution, re‐translocation and homeostasis of inorganic phosphate in barley leavesPlant, Cell & Environment, 1996
- Carbonic Anhydrase Activity in Leaves of Wheat Genotypes Differing in Zn EfficiencyJournal of Plant Physiology, 1995
- Compartmental analysis of35SO2-4exchange kinetics in roots and leaves of a tropical legume Macroptilium atropurpureum cv. SiratroJournal of Experimental Botany, 1994
- Superoxide DismutasesPlant Physiology, 1977
- Effect of Zinc Nutrition on Photosynthesis and Carbonic Anhydrase Activity in CottonPhysiologia Plantarum, 1976
- A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye BindingAnalytical Biochemistry, 1976
- A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye bindingAnalytical Biochemistry, 1976