Suppression of fibroblast and bacterial adhesion by MPC coating on acrylic intraocular lenses
- 1 May 2006
- journal article
- Published by Ovid Technologies (Wolters Kluwer Health) in Journal of Cataract & Refractive Surgery
- Vol. 32 (5), 859-866
- https://doi.org/10.1016/j.jcrs.2006.02.010
Abstract
To investigate cell adhesion to intraocular lens (IOL) surfaces having different properties using bacteria and fibroblasts. Department of Ophthalmology, Tokyo Medical University Hospital, Tokyo, Japan. The polished acrylic IOLs VA60-BB and VA-60-CB (Hoya) were used with no coating or after coating with poly(2-methacryloyloxyethyl phosphocholine-co-n-butyl-methacrylate) (MPC) or methane gas plasma. Each IOL was placed in a culture of Staphylococcus aureus (5 ×107 colony forming units [CFU]/mL) or fibroblasts (SUSM-1, 1 × 104 cells/mL), and the cells adhering to the IOL surface were counted after the culture. Fibroblast adhesiveness was evaluated by centrifuging post-culture IOLs in test tubes at up to 15 000 rpm and looking at the ICAM-1 mRNA expression in the adhering fibroblasts using real-time polymerase chain reaction. After 1 minute in the bacterial culture, the mean adhering bacteria count was 0.7 × 106 CFU in the MPC-coated IOL group and about 2.0 × 106 CFU in the noncoated IOL group (P = 0.03) and the plasma-coated IOL group (P = 0.02). After 96 hours in the fibroblast culture, the adhering fibroblast count was 2.2/mm2 in the MPC-coated IOL group, significantly lower (P = 0.009) than 57.6/mm2 in the noncoated IOL group and 125.8/mm2 in the plasma-coated IOL group. Cell adhesion and ICAM-1 mRNA expression were weak on the MPC-coated IOL, intermediate on the noncoated IOL, and relatively strong on the plasma-coated IOL. The MPC-coated IOL surface inhibited bacterial and fibroblast adhesion, which is an initial stage of cell proliferation and expression, suggesting that MPC coating may provide an effective means of reducing the risk for endophthalimitis in IOL implantation.Keywords
This publication has 21 references indexed in Scilit:
- Cell adhesion to acrylic intraocular lens associated with lens surface propertiesJournal of Cataract & Refractive Surgery, 2005
- Suppression of the inflammatory response from adherent cells on phospholipid polymersJournal of Biomedical Materials Research Part A, 2003
- Surface roughness of intraocular lenses and inflammatory cell adhesion to lens surfacesJournal of Cataract & Refractive Surgery, 2003
- Effect of vancomycin on Staphylococcus epidermidis adherence to poly(methyl methacrylate) intraocular lensesJournal of Cataract & Refractive Surgery, 2002
- Physicochemical Surface Properties of Various Intraocular LensesOphthalmic Research, 2001
- Inhibition of fibroblast cell adhesion on substrate by coating with 2-methacryloyloxyethyl phosphorylcholine polymersJournal of Biomaterials Science, Polymer Edition, 1999
- Why do phospholipid polymers reduce protein adsorption?Journal of Biomedical Materials Research, 1998
- Plasma treatments and plasma deposition of polymers for biomedical applicationsSurface and Coatings Technology, 1998
- Inflammatory Mediators in Postoperative Aphakic and Pseudophakic Baboon EyesAmerican Journal of Ophthalmology, 1990
- Preparation of Phospholipid Polylners and Their Properties as Polymer Hydrogel MembranesPolymer Journal, 1990