Parallel Picoliter RT-PCR Assays Using Microfluidics
- 4 January 2006
- journal article
- Published by American Chemical Society (ACS) in Analytical Chemistry
- Vol. 78 (3), 956-958
- https://doi.org/10.1021/ac0513865
Abstract
The development of microfluidic tools for high-throughput nucleic acid analysis has become a burgeoning area of research in the post-genome era. Here, we have developed a microfluidic chip to perform 72 parallel 450-pL RT-PCRs. We took advantage of Taqman hydrolysis probe chemistry to detect RNA templates as low as 34 copies. The device and method presented here may enable highly parallel single cell gene expression analysis.Keywords
This publication has 7 references indexed in Scilit:
- Miniaturised nucleic acid analysisLab on a Chip, 2004
- Continuous-flow DNA and RNA amplification chip combined with laser-induced fluorescence detectionAnalytica Chimica Acta, 2003
- Microfabricated Device for DNA and RNA Amplification by Continuous-Flow Polymerase Chain Reaction and Reverse Transcription-Polymerase Chain Reaction with Cycle Number SelectionAnalytical Chemistry, 2002
- Absolute quantification of mRNA using real-time reverse transcription polymerase chain reaction assaysJournal of Molecular Endocrinology, 2000
- A miniature integrated device for automated multistep genetic assaysNucleic Acids Research, 2000
- Monolithic Microfabricated Valves and Pumps by Multilayer Soft LithographyScience, 2000
- Three abundance classes in HeLa cell messenger RNANature, 1974