Brown Planthopper (N. lugens Stal) Feeding Behaviour on Rice Germplasm as an Indicator of Resistance
Open Access
- 14 July 2011
- journal article
- research article
- Published by Public Library of Science (PLoS) in PLOS ONE
- Vol. 6 (7), e22137
- https://doi.org/10.1371/journal.pone.0022137
Abstract
The brown planthopper (BPH) Nilaparvata lugens (Stal) is a serious pest of rice in Asia. Development of novel control strategies can be facilitated by comparison of BPH feeding behaviour on varieties exhibiting natural genetic variation, and then elucidation of the underlying mechanisms of resistance. BPH feeding behaviour was compared on 12 rice varieties over a 12 h period using the electrical penetration graph (EPG) and honeydew clocks. Seven feeding behaviours (waveforms) were identified and could be classified into two phases. The first phase involved patterns of sieve element location including non penetration (NP), pathway (N1+N2+N3), xylem (N5) [21] and two new feeding waveforms, derailed stylet mechanics (N6) and cell penetration (N7). The second feeding phase consisted of salivation into the sieve element (N4-a) and sieve element sap ingestion (N4-b). Production of honeydew drops correlated with N4-b waveform patterns providing independent confirmation of this feeding behaviour. Overall variation in feeding behaviour was highly correlated with previously published field resistance or susceptibility of the different rice varieties: BPH produced lower numbers of honeydew drops and had a shorter period of phloem feeding on resistant rice varieties, but there was no significant difference in the time to the first salivation (N4-b). These qualitative differences in behaviour suggest that resistance is caused by differences in sustained phloem ingestion, not by phloem location. Cluster analysis of the feeding and honeydew data split the 12 rice varieties into three groups: susceptible, moderately resistant and highly resistant. The screening methods that we have described uncover novel aspects of the resistance mechanism (or mechanisms) of rice to BPH and will in combination with molecular approaches allow identification and development of new control strategies.Keywords
This publication has 40 references indexed in Scilit:
- The species of the genus Oryza and transfer of useful genes from wild species into cultivated rice, O. sativaBreeding Science, 2010
- Assessment of the impact of insecticides on Anagrus nilaparvatae (Pang et Wang) (Hymenoptera: Mymanidae), an egg parasitoid of the rice planthopper, Nilaparvata lugens (Hemiptera: Delphacidae)Crop Protection, 2008
- Herbivore-Induced Callose Deposition on the Sieve Plates of Rice: An Important Mechanism for Host ResistancePlant Physiology, 2008
- Mapping and Progress toward Map‐Based Cloning of Brown Planthopper Biotype‐4 Resistance Gene Introgressed from Oryza officinalis into Cultivated Rice, O. sativaCrop Science, 2002
- Molecular tagging of genes for brown planthopper resistance and earliness introgressed from Oryza australiensis into cultivated rice, O. sativaGenome, 1994
- Effects on the feeding behaviour of Nilaparvata lugens (Stål) of sublethal concentrations of the foliarly applied nitromethylene heterocycle 2‐nitromethylene‐1, 3‐thiazinan‐3‐yl‐carbamaldehydePhysiological Entomology, 1993
- THE RICE BROWN PLANTHOPPER: Feeding Physiology and Host Plant InteractionsAnnual Review of Entomology, 1982
- Genetics of Resistance to Brown Planthoppers and Green Leafhoppers in Oryza sativa L.1Crop Science, 1971
- Hierarchical Grouping to Optimize an Objective FunctionJournal of the American Statistical Association, 1963
- HIERARCHICAL GROUPING TO OPTIMIZE AN OBJECTIVE FUNCTIONJournal of the American Statistical Association, 1962