Physiological and morphological characterization of parvalbumin‐containing interneurons of the rat basolateral amygdala
- 19 July 2006
- journal article
- research article
- Published by Wiley in Journal of Comparative Neurology
- Vol. 498 (1), 142-161
- https://doi.org/10.1002/cne.21049
Abstract
The basolateral amygdala (BLA) is critical for the generation of emotional behavior and the formation of emotional memory. Understanding the neuronal mechanisms that contribute to emotional information processing in the BLA will ultimately require knowledge of the anatomy and physiology of its constituent neurons. Two major cell classes exist in the BLA, pyramidal projection neurons and nonpyramidal interneurons. Although the properties of projection neurons have been studied in detail, little is known about the properties of BLA interneurons. We have used whole-cell patch clamp recording techniques to examine the physiological properties of 48 visually identified putative interneurons from the rat anterior basolateral amygdalar nucleus. Here, we report that BLA interneurons can be differentiated into four electrophysiologically distinct subtypes based on their intrinsic membrane properties and their response to afferent synaptic input. Interneuron subtypes were named according to their characteristic firing pattern generated in response to transient depolarizing current injection and were grouped as follows: 1) burst-firing interneurons (n = 13), 2) regular-firing interneurons (n = 11), 3) fast-firing interneurons (n = 10), and 4) stutter-firing interneurons (n = 14). Post hoc histochemical visualization confirmed that all 48 recorded neurons had morphological properties consistent with their being local circuit interneurons. Moreover, by using triple immunofluorescence (for biocytin, calcium-binding proteins, and neuropeptides) in conjunction with patch clamp recording, we further demonstrated that over 60% of burst-firing and stutter-firing interneurons also expressed the calcium-binding protein parvalbumin (PV+). These data demonstrate that interneurons of the BLA show both physiological and neurochemical diversity. Moreover, we demonstrate that the burst- and stutter-firing patterns positively correlate with PV+ immunoreactivity, suggesting that these neurons may represent functionally distinct subpopulations. J. Comp. Neurol. 498:142–161, 2006.Keywords
This publication has 111 references indexed in Scilit:
- Correlation Maps Allow Neuronal Electrical Properties to be Predicted from Single-cell Gene Expression Profiles in Rat NeocortexCerebral Cortex, 2004
- Synaptic connections of distinct interneuronal subpopulations in the rat basolateral amygdalar nucleusJournal of Comparative Neurology, 2003
- Cortical Microcircuits: Diverse or Canonical?Neuron, 2002
- Anatomical, Physiological, Molecular and Circuit Properties of Nest Basket Cells in the Developing Somatosensory CortexCerebral Cortex, 2002
- Resonate-and-fire neuronsNeural Networks, 2001
- Cell surface domain specific postsynaptic currents evoked by identified GABAergic neurones in rat hippocampus in vitroThe Journal of Physiology, 2000
- Glutamate and aspartate immunoreactive neurons of the rat basolateral amygdala: Colocalization of excitatory amino acids and projections to the limbic circuitJournal of Comparative Neurology, 1996
- Immunocytochemical localization of calretinin in the forebrain of the ratJournal of Comparative Neurology, 1991
- Neurons of the lateral and basolateral amygdaloid nuclei: A golgi study in the ratJournal of Comparative Neurology, 1982
- Neurons of the basolateral amygdala: A Golgi study in the opossum (Didelphis virginiana)Journal of Anatomy, 1981