Epitopes on β2-GPI recognized by anticardiolipin antibodies
- 1 February 1998
- journal article
- review article
- Published by SAGE Publications in Lupus
- Vol. 7 (2_suppl), 14-17
- https://doi.org/10.1177/096120339800700204
Abstract
Anticardiolipin antibodies (aCL) found in sera from patients with antiphospholipid syndrome recognize a cryptic epitope that appears on the β2-glycoprotein I (β2-GPI) molecule when β2-GPI interacts with a lipid membrane composed of negatively charged phospholipid or when β2-GPI is adsorbed on a polyoxygenated polystyrene plate. A homology based model of β2-GPI was constructed based on the NMR coordinates of sushi domains of human factor H. The conformation was like a cylinder consisting of five domains, its IV and V domains being glued by electrostatic interaction. We used phage-displayed random peptide libraries to search the epitopes of human aCL. Structures similar to consensus sequences selected by a biopanning method was found on domain IV of β2-GPI.Keywords
This publication has 25 references indexed in Scilit:
- Molecular Studies on Phospholipid-Binding Sites and Cryptic Epitopes Appearing on β2-Glycoprotein I Structure Recognized by Anticardiolipin AntibodiesLupus, 1995
- Activity, disulphate mapping and structural modelling of the fifth domain of human β2‐glycoprotein IFEBS Letters, 1992
- Amino acid sequence and location of the disulfide bonds in bovine .beta.2 glycoprotein I: the presence of five Sushi domainsBiochemistry, 1991
- Molecular definition of human β2-glycoprotein (α2-GPI) by cDNA cloning and inter-species differences of β2-GPI in alternation of anticardiolipin bindingInternational Immunology, 1991
- Three-dimensional structure of a complement control protein module in solutionJournal of Molecular Biology, 1991
- Anticardiolipin antibodies (ACA) directed not to cardiolipin but to a plasma protein cofactorThe Lancet, 1990
- β2-glycoprotein-I (apo-H) inhibits the release reaction of human platelets during ADP-induced aggregationAtherosclerosis, 1987
- Prothrombinase activity of human platelets is inhibited by β2-glycoprotein-IBiochimica et Biophysica Acta (BBA) - General Subjects, 1986
- CHARMM: A program for macromolecular energy, minimization, and dynamics calculationsJournal of Computational Chemistry, 1983
- The binding of β2‐glycoprotein‐I to human serum lipoproteinsFEBS Letters, 1979