Drug Treatment of Cancer Cell Lines: A Way to Select for Cancer Stem Cells?
Open Access
- 4 March 2011
- Vol. 3 (1), 1111-1128
- https://doi.org/10.3390/cancers3011111
Abstract
Tumors are generally composed of different cell types. In recent years, it has been shown that in many types of cancers a subset of cells show peculiar characteristics, such as the ability to induce tumors when engrafted into host animals, self-renew and being immortal, and give rise to a differentiated progeny. These cells have been defined as cancer stem cells (CSCs) or tumor initiating cells. CSCs can be isolated both from tumor specimens and established cancer cell lines on the basis of their ability to exclude fluorescent dyes, express specific cell surface markers or grow in particular culture conditions. A key feature of CSCs is their resistance to chemotherapeutic agents, which could contribute to the remaining of residual cancer cells after therapeutic treatments. It has been shown that CSC-like cells can be isolated after drug treatment of cancer cell lines; in this review, we will describe the strategies so far applied to identify and isolate CSCs. Furthermore, we will discuss the possible use of these selected populations to investigate CSC biology and develop new anticancer drugs.This publication has 108 references indexed in Scilit:
- Molecular cytogenetic characterization of stem-like cancer cells isolated from established cell linesCancer Letters, 2010
- Targeting poly(ADP-ribose) polymerase activity for cancer therapyCellular and Molecular Life Sciences, 2010
- Curcumin inhibits the side population (SP) phenotype of the rat C6 glioma cell line: Towards targeting of cancer stem cells with phytochemicalsCancer Letters, 2010
- Identification of cancer stem cell-like cells from human epithelial ovarian carcinoma cell lineCellular and Molecular Life Sciences, 2010
- Cancer Stem Cells: Controversial or Just Misunderstood?Cell Stem Cell, 2009
- The Epithelial-Mesenchymal Transition Generates Cells with Properties of Stem CellsCell, 2008
- ALDH1 Is a Marker of Normal and Malignant Human Mammary Stem Cells and a Predictor of Poor Clinical OutcomeCell Stem Cell, 2007
- Induction of Pluripotent Stem Cells from Adult Human Fibroblasts by Defined FactorsCell, 2007
- NF-κB pathway inhibitors preferentially inhibit breast cancer stem-like cellsBreast Cancer Research and Treatment, 2007
- Induction of Pluripotent Stem Cells from Mouse Embryonic and Adult Fibroblast Cultures by Defined FactorsCell, 2006