MicroRNAs 17-5p–20a–106a control monocytopoiesis through AML1 targeting and M-CSF receptor upregulation
- 24 June 2007
- journal article
- research article
- Published by Springer Science and Business Media LLC in Nature
- Vol. 9 (7), 775-787
- https://doi.org/10.1038/ncb1613
Abstract
We investigated the role of microRNAs (miRNA) 17-5p, 20a and 106a in monocytic differentiation and maturation. In unilineage monocytic culture generated by haematopoietic progenitor cells these miRNAs are downregulated, whereas the transcription factor acute myeloid leukaemia-1 (AML1; also known as Runt-related transcription factor 1, Runx1) is upregulated at protein but not mRNA level. As miRNAs 17-5p, 20a and 106a bind the AML1 mRNA 3′UTR, their decline may unblock AML1 translation. Accordingly, transfection with miRNA 17-5p–20a–106a suppresses AML1 protein expression, leading to M-CSF receptor (M-CSFR) downregulation, enhanced blast proliferation and inhibition of monocytic differentiation and maturation. Treatment with anti-miRNA 17-5p, 20a and 106a causes opposite effects. Knockdown of AML1 or M-CSFR by short interfering RNA (siRNA) mimics the action of the miRNA 17-5p–20a–106a, confirming that these miRNAs target AML1, which promotes M-CSFR transcription. In addition, AML1 binds the miRNA 17-5p–92 and 106a–92 cluster promoters and transcriptionally inhibits the expression of miRNA 17-5p–20a–106a. These studies indicate that monocytopoiesis is controlled by a circuitry involving sequentially miRNA 17-5p–20a–106a, AML1 and M-CSFR, whereby miRNA 17-5p–20a–106a function as a master gene complex interlinked with AML1 in a mutual negative feedback loop.Keywords
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