The microorganisms in chronically infected end-stage and non-end-stage cystic fibrosis patients
- 1 July 2012
- journal article
- Published by Oxford University Press (OUP) in FEMS Immunology & Medical Microbiology
- Vol. 65 (2), 236-244
- https://doi.org/10.1111/j.1574-695x.2011.00925.x
Abstract
Patients suffering from cystic fibrosis (CF) develop chronic lung infections because of highly viscous mucus, where bacteria can form biofilms. In this study, we investigated the microorganisms present in the lungs of end-stage and non-end-stage patients using standard culturing techniques and molecular methods. Tissue and sputum samples (n = 34) from explanted lungs of five end-stage patients were examined along with routine expectorates (n = 15) from 13 patients with non-end-stage CF, representing earlier stages of chronic lung infections. Previously, using peptide nucleic acid (PNA) fluorescence in situ hybridization (FISH), we have shown that Pseudomonas aeruginosa was the sole pathogen in end-stage CF lungs (Pediatr Pulmonol 2009, 44: 547). In this study, this tendency was supported by the results of real-time PCR, confirming previous results obtained by standard culturing and 16S rRNA gene analysis (J Clin Microbiol 2011, 49: 4352). Conversely, the non-end-stage patients were found to harbor several species by culturing. PNA FISH confirmed heterogeneous microbiota and showed that the bacteria were located in monospecies aggregates with no apparent physical interaction between the different microcolonies. In conclusion, standard culturing identifies the dominating pathogens, which seem to reside in monospecies microcolonies. The possibility of signaling between the distinct microcolonies still has to be verified and elucidated.Keywords
This publication has 34 references indexed in Scilit:
- True Microbiota Involved in Chronic Lung Infection of Cystic Fibrosis Patients Found by Culturing and 16S rRNA Gene AnalysisJournal of Clinical Microbiology, 2011
- Spatial distribution of microbial communities in the cystic fibrosis lungThe ISME Journal, 2011
- Phylogenetic and metabolic diversity of bacteria associated with cystic fibrosisThe ISME Journal, 2010
- Nonrandom Distribution of Pseudomonas aeruginosa and Staphylococcus aureus in Chronic WoundsJournal of Clinical Microbiology, 2009
- Comparison of the sensitivity of culture, PCR and quantitative real-time PCR for the detection of Pseudomonas aeruginosain sputum of cystic fibrosis patientsBMC Microbiology, 2009
- Identification of Pseudomonas aeruginosa by a duplex real-time polymerase chain reaction assay targeting the ecfX and the gyrB genesDiagnostic Microbiology and Infectious Disease, 2009
- Novel experimental Pseudomonas aeruginosa lung infection model mimicking long‐term host–pathogen interactions in cystic fibrosisAPMIS, 2009
- Survey of bacterial diversity in chronic wounds using Pyrosequencing, DGGE, and full ribosome shotgun sequencingBMC Microbiology, 2008
- Why chronic wounds will not heal: a novel hypothesisWound Repair and Regeneration, 2007
- Use of 16S rRNA Gene Profiling by Terminal Restriction Fragment Length Polymorphism Analysis To Compare Bacterial Communities in Sputum and Mouthwash Samples from Patients with Cystic FibrosisJournal of Clinical Microbiology, 2006