An antimicrobial effect associated with rabbit primary aqueous humour.
Open Access
- 1 February 1994
- journal article
- research article
- Published by BMJ in British Journal of Ophthalmology
- Vol. 78 (2), 142-148
- https://doi.org/10.1136/bjo.78.2.142
Abstract
Viability curves were recorded for a variety of bacteria suspended in rabbit primary aqueous humour. Controls included neat rabbit serum, dilute rabbit serum (1/50), dilute heat treated fetal bovine serum (1/50), and balanced salt solution. Rabbit aqueous humour was bactericidal in vitro for Staphylococcus aureus and Pseudomonas aeruginosa. Micrococcus spp, Streptococcus pneumoniae and Escherichia coli were not affected. The factor mediating the bactericidal effect survives filtration (0.2 micron porosity) and freezing in liquid nitrogen (-196 degrees C) but is labile when stored in air at temperatures of 4 degrees C, 25 degrees C, and 37 degrees C for 1 hour. The bactericidal effect was stable when aqueous humour was stored in an atmosphere of 5% CO2 at 37 degrees C for 3 hours. The antibacterial component in rabbit aqueous humour has yet to be identified.Keywords
This publication has 28 references indexed in Scilit:
- Endophthalmitis following extracapsular cataract surgery: a review of 32 cases.British Journal of Ophthalmology, 1992
- Insect antibacterial proteins: Not just for insects and against bacteriaBioEssays, 1991
- Randomised single blind trial to compare the toxicity of subconjunctival gentamicin and cefuroxime in cataract surgery.British Journal of Ophthalmology, 1990
- Bacterial contamination of intraocular and extraocular fluids during extracapsular cataract extractionEye, 1989
- A retrospective review of endophthalmitis due to coagulase-negative staphylococci.British Journal of Ophthalmology, 1985
- Effect of inoculum size on the induction of endophthalmitis in aphakic rabbit eyesActa Ophthalmologica, 1985
- Bacterial contamination of intraocular lens surgery.British Journal of Ophthalmology, 1984
- Immunoglobulins in human aqueous humour.British Journal of Ophthalmology, 1977
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970
- The estimation of the bactericidal power of the bloodEpidemiology and Infection, 1938