T-cell antigen CD28 mediates adhesion with B cells by interacting with activation antigen B7/BB-1.
- 1 July 1990
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 87 (13), 5031-5035
- https://doi.org/10.1073/pnas.87.13.5031
Abstract
Studies using monoclonal antibodies (mAbs) have implicated the homodimeric glycoprotein CD28 as an important regulator of human T-cell activation, in part by posttranscriptional control of cytokine mRNA levels. Although the CD28 antigen has functional and structural characteristics of a receptor, a natural ligand for this molecule has not been identified. Here we show that the CD28 antigen, expressed in Chinese hamster overy (CHO) cells, mediated specific intercellular adhesion with human lymphoblastoid and leukemic B cell lines and with activated primary murine B cells. CD28-mediated adhesion wasnot dependent upon divalent cations. Several mAbs were identifed that inhibited CD28-mediated adhesion, including mAbs BB-1 against the B-cell activation antigen B7/BB-1 in some mAbs against major histocompatibility complex class I antigens. B7/BB-1 expression correlated closely with Cd28-mediated ahesion, but class I expression did not. Transfected COS cells expressing the B7/BB-1 antigen adhered to CD28+ CHO cells; this adhesion was blocked by mAbs to CD28 and B7/BB-1. The specific recognition by CD28 of the B-cell activation antigen B7/BB-1 represents a heterophilic interaction between members of the immunoglobulin superfamily that may serve to regulate T-cell cytokine levels at sites of B-cell activation.This publication has 36 references indexed in Scilit:
- Role of the CD28 receptor in T-cell activationImmunology Today, 1990
- Identification and characterization of the T lymphocyte adhesion receptor for an alternative cell attachment domain (CS-1) in plasma fibronectin.The Journal of cell biology, 1989
- Signal transduction in lymphocyte activation through crosslinking of HLA class I moleculesHuman Immunology, 1989
- Adhesive protein receptors on hematopoietic cellsImmunology Today, 1988
- Cell-cell adhesion mediated by CD8 and MHC class I moleculesNature, 1988
- The Immunoglobulin Superfamily—Domains for Cell Surface RecognitionAnnual Review of Immunology, 1988
- LymphokinesNew England Journal of Medicine, 1987
- The Lymphocyte Function Associated LFA-1, CD2, and LFA-3 Molecules: Cell Adhesion Receptors of the Immune SystemAnnual Review of Immunology, 1987
- Polypeptides on human B lymphocytes associated with cell activationHuman Immunology, 1986
- T90/44 (9.3 antigen). A cell surface molecule with a function in human T cell activationEuropean Journal of Immunology, 1986