Upconversion Luminescence-Activated DNA Nanodevice for ATP Sensing in Living Cells
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Open Access
- 6 January 2018
- journal article
- research article
- Published by American Chemical Society (ACS) in Journal of the American Chemical Society
- Vol. 140 (2), 578-581
- https://doi.org/10.1021/jacs.7b11161
Abstract
Designer DNA nanodevices have attracted extensive interest for detection of specific targets in living cells. However, it still re-mains a great challenge to construct DNA sensing devices that can be activated at desired time with a remotely applied stimulus. Here we report a rationally designed, synthetic DNA nanodevice that can detect ATP in living cells in a upconversion luminescence-activatable manner. The nanodevice consists of a UV light activatable aptamer probe and lanthanide-doped upconversion nanoparticles which acts as the nanotransducers to operate the device in response to NIR light. We demonstrate that the nanodevice not only enables efficient cellular delivery of the aptamer probe into live cells, but also allows the temporal control over its fluorescent sensing activity for ATP by NIR light irradiation in vitro and in vivo. Ultimately, with the availability of diverse aptamers selected in vitro, the DNA nanodevice platform will allow NIR-triggered sensing of various targets as well as modulation of biological functions in living systems.Keywords
Funding Information
- National Natural Science Foundation of China (21771044)
- Central People's Government of the People's Republic of China
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