Rapid Genotypic Detection of Rifampin- and Isoniazid-Resistant Mycobacterium tuberculosis Directly in Clinical Specimens
- 1 July 2006
- journal article
- Published by American Society for Microbiology in Journal of Clinical Microbiology
- Vol. 44 (7), 2605-2608
- https://doi.org/10.1128/jcm.00752-06
Abstract
A multiplex PCR DNA strip assay (Genotype MTBDR) designed to detect rifampin ( rpoB ) and high-level isoniazid ( katG ) resistance mutations in Mycobacterium tuberculosis isolates was optimized for clinical specimens. Successful genotypic results were achieved with 36 of 38 (95%) smear-positive respiratory specimens, allowing rapid therapeutic adjustments in transmittable drug-resistant tuberculosis.Keywords
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