Variant of hepatitis B virus isolated in zimbabwe
- 1 January 1994
- journal article
- Published by Wiley in Journal of Medical Virology
- Vol. 42 (1), 73-78
- https://doi.org/10.1002/jmv.1890420114
Abstract
The polymerase chain reaction (PCR) was used to amplify an approximately 1.2 kb DNA fragment encompassing the pre‐S/S gene region of HBV DNA from serum of patients with acute hepatitis B virus infection. Nucleotide sequence analysis revealed a number of interesting features in the S gene region. Two Bam HI sites were located at nucleotide positions 557 and 872, respectively, in the S gene. Guanine (G) was found at nucleotide position 903 as part of AGA, the codon for arginine (R) corresponding to amino acid position 122 of the S protein. Ade‐nine (A) was found at nucleotide position 1017 as part of AXIA, the codon for lysine (K) corresponding to amino acid position 160 of the S protein. Nucleotide sequence alignment revealed a 97% homology to the corresponding domain of an HBVadw genome (clone pFDW294). Within the second loop of the “a” determinant, two mutations resulting in substitution of serine or threonine with the hydrophobic amino acids, methio‐nine at position 143 and with alanine in place of glycine at position 145, are predicted from the consensus nucleotide sequence of the PCR‐derived clones. Subtyping with monoclonal antibodies showed that the HBsAg was of the ayw subtype. © 1994 Wiiey‐Liss, Inc.Keywords
This publication has 16 references indexed in Scilit:
- Mutations within the S Gene of Hepatitis B Virus Transmitted from Mothers to Babies Immunized with Hepatitis B Immune Globulin and VaccinePediatric Research, 1992
- Gly145 to Arg substitution in HBs antigen of immune escape mutant of hepatitis B virusBiochemical and Biophysical Research Communications, 1992
- Comparison of the amino acid sequences of nine different serotypes of hepatitis B surface antigen and genomic classification of the corresponding hepatitis B virus strainsJournal of General Virology, 1992
- Independent emergence of a vaccine-induced escape mutant of hepatitis B virusJournal of Hepatology, 1991
- Vaccine-induced escape mutant of hepatitis B virusThe Lancet, 1990
- Characterization and biological properties of a hepatitis B virus isolated from a patient without hepatitis B virus serologic markersHepatology, 1990
- Discrimination of hepatitis B virus (HBV) subtypes using monoclonal antibodies to the PreS1 and PreS2 domains of the viral envelopeVirology, 1990
- Mutants of the hepatitis b virus surface antigen that define some antigenically essential residues in the immunodominant a regionJournal of Medical Virology, 1989
- Typing Hepatitis B Virus by Homology in Nucleotide Sequence: Comparison of Surface Antigen SubtypesJournal of General Virology, 1988
- An oligonucleotide probe for the detection of hepatitis B virus DNA in serumJournal of Virological Methods, 1987