Disuse atrophy and exercise rehabilitation in humans profoundly affects the expression of genes associated with the regulation of skeletal muscle mass
- 14 April 2004
- journal article
- clinical trial
- Published by Wiley in The FASEB Journal
- Vol. 18 (9), 1025-1027
- https://doi.org/10.1096/fj.03-1228fje
Abstract
Skeletal muscle atrophy occurs as a consequence of injury, illness, surgery, and muscle disuse, impacting appreciably on health care costs and patient quality of life, particularly in the absence of appropriate rehabilitation. The molecular mechanisms that regulate muscle mass during atrophy and rehabilitation in humans have not been elucidated, despite several robust candidate pathways being identified. Here, we induced skeletal muscle atrophy in healthy volunteers using two weeks of limb immobilization, and then stimulated the restoration of muscle mass with six weeks of supervised exercise rehabilitation. We determined muscle mass and function and performed targeted gene expression analysis at prescribed time points during immobilization and rehabilitation. For the first time, we have identified novel changes in gene expression following immobilization-induced atrophy and during a program of rehabilitative exercise that restored muscle mass and function. Furthermore, we have shown that exercise performed immediately following immobilization induces profound changes in the expression of a number of genes in favor of the restoration of muscle mass, within 24 h. This information will be of considerable importance to our understanding of how immobilization and contraction stimulate muscle atrophy and hypertrophy, respectively, and to the development of novel therapeutic strategies aimed at maintaining or restoring muscle mass.Keywords
This publication has 47 references indexed in Scilit:
- Signalling pathways that mediate skeletal muscle hypertrophy and atrophyNature, 2003
- Increased transcription of ubiquitin–proteasome system components: molecular responses associated with muscle atrophyThe International Journal of Biochemistry & Cell Biology, 2003
- Exercise treatment to counteract protein wasting of chronic diseasesCurrent Opinion in Clinical Nutrition and Metabolic Care, 2003
- Expression of a calpastatin transgene slows muscle wasting and obviates changes in myosin isoform expression during murine muscle disuseThe Journal of Physiology, 2002
- Molecular adaptations of neuromuscular disease‐associated proteins in response to eccentric exercise in human skeletal muscleThe Journal of Physiology, 2002
- Oral creatine supplementation facilitates the rehabilitation of disuse atrophy and alters the expression of muscle myogenic factors in humansThe Journal of Physiology, 2001
- Effects of a 12-wk resistance exercise program on skeletal muscle strength in children with burn injuriesJournal of Applied Physiology, 2001
- Relationship of skeletal muscle mass, muscle strength and bone mineral density in adults with cystic fibrosisClinical Science, 2000
- Wasting in CancerJournal of Nutrition, 1999
- The calpain system and skeletal muscle growthCanadian Journal of Animal Science, 1998