Development of a 16S rRNA Gene Primer and PCR-Restriction Fragment Length Polymorphism Method for Rapid Detection of Members of the Genus Megasphaera and Species-Level Identification
- 1 August 2011
- journal article
- Published by American Society for Microbiology in Applied and Environmental Microbiology
- Vol. 77 (15), 5533-5535
- https://doi.org/10.1128/aem.00359-11
Abstract
The genus Megasphaera is relevant to the environment, human health and food, and renewable energy for the future. In this study, a primer set was designed for PCR-restriction fragment length polymorphism (RFLP) analyses to detect and identify the members of Megasphaera . Direct detection and identification were achieved for environmental samples and isolates.Keywords
This publication has 9 references indexed in Scilit:
- Development of a simple bio-hydrogen production system through dark fermentation by using unique microfloraInternational Journal of Hydrogen Energy, 2010
- Hydrogen: the future energy carrierPhilosophical Transactions of the Royal Society A: Mathematical, Physical and Engineering Sciences, 2010
- Phylogenetic and physiological characterization of mesophilic and thermophilic bacteria from a sewage sludge composting process in Sapporo, JapanWorld Journal of Microbiology and Biotechnology, 2010
- Megasphaera paucivorans sp. nov., Megasphaera sueciensis sp. nov. and Pectinatus haikarae sp. nov., isolated from brewery samples, and emended description of the genus PectinatusInternational Journal of Systematic and Evolutionary Microbiology, 2006
- Pectinatus, Megasphaera and ZymophilusPublished by Springer Science and Business Media LLC ,2006
- Megasphaera elsdenii JCM1772T Normalizes Hyperlactate Production in the Large Intestine of Fructooligosaccharide-Fed Rats by Stimulating Butyrate ProductionJournal of Nutrition, 2003
- Phylogenetic analysis of some Sporomusa sub-branch members isolated from human clinical specimens: description of Megasphaera micronuciformis sp. nov.International Journal of Systematic and Evolutionary Microbiology, 2003
- The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis toolsNucleic Acids Research, 1997
- Megasphaera cerevisiae sp. nov.: A New Gram-negative Obligately Anaerobic Coccus Isolated from Spoiled BeerSystematic and Applied Microbiology, 1985