Isolation of Methyl Parathion-Degrading Strain M6 and Cloning of the Methyl Parathion Hydrolase Gene
Open Access
- 1 October 2001
- journal article
- Published by American Society for Microbiology in Applied and Environmental Microbiology
- Vol. 67 (10), 4922-4925
- https://doi.org/10.1128/aem.67.10.4922-4925.2001
Abstract
A degradative bacterium, M6, was isolated and presumptively identified as Plesiomonas sp. strain M6 was able to hydrolyze methyl parathion to p -nitrophenol. A novel organophosphate hydrolase gene designated mpd was selected from its genomic library prepared by shotgun cloning. The nucleotide sequence of the mpd gene was determined. The gene could be effectively expressed in Esherichia coli .Keywords
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