Capture of heat-killed Mycobacterium bovis bacillus Calmette-Guerin by intelectin-1 deposited on cell surfaces
- 29 January 2009
- journal article
- Published by Oxford University Press (OUP) in Glycobiology
- Vol. 19 (5), 518-526
- https://doi.org/10.1093/glycob/cwp013
Abstract
Intelectin is an extracellular animal lectin found in chordata. Although human and mouse intelectin-1 recognize galactofuranosyl residues included in cell walls of various microorganisms, the physiological function of mammalian intelectin had been unclear. In this study, we found that human intelectin-1 was a serum protein and bound to Mycobacterium bovis bacillus Calmette-Guérin (BCG). Human intelectin-1-binding to BCG was inhibited by Ca2+-depletion, galactofuranosyl disaccharide, ribose, or xylose, and was dependent on the trimeric structure of human intelectin-1. Although monomeric, mouse intelectin-1 bound to BCG, with its C-terminal region contributing to efficient binding. Human intelectin-1-transfected cells not only secreted intelectin-1 into culture supernatant but also expressed intelectin-1 on the cell surface. The cell surface intelectin-1 was not a glycosylphosphatidylinositol-anchored membrane protein. Intelectin-1-transfected cells captured BCG more than untransfected cells, and the BCG adherence was inhibited by an inhibitory saccharide of intelectin-1. Intelectin-1-preincubated cells took up BCG more than untreated cells, but the adhesion of intelectin-1-bound BCG was the same as that of untreated BCG. Mouse macrophages phagocytosed BCG more efficiently in medium containing mouse intelectin-1 than in control medium. These results indicate that intelectin is a host defense lectin that assists phagocytic clearance of microorganisms.Keywords
This publication has 35 references indexed in Scilit:
- Identification, cloning and tissue localization of a rainbow trout (Oncorhynchus mykiss) intelectin-like protein that binds bacteria and chitinFish & Shellfish Immunology, 2008
- Nippostrongylus brasiliensis: Identification of intelectin-1 and -2 as Stat6-dependent genes expressed in lung and intestine during infectionExperimental Parasitology, 2007
- Pulmonary collectins in innate immunity of the lungCellular Microbiology, 2007
- Differential structure and activity between human and mouse intelectin-1: Human intelectin-1 is a disulfide-linked trimer, whereas mouse homologue is a monomerGlycobiology, 2007
- Dissecting asthma using focused transgenic modeling and functional genomicsJournal of Allergy and Clinical Immunology, 2005
- Galectin-4 and sulfatides in apical membrane trafficking in enterocyte-like cellsThe Journal of cell biology, 2005
- Type II Alveolar Cells Play Roles in Macrophage‐Mediated Host Innate Resistance to Pulmonary Mycobacterial Infections by Producing Proinflammatory CytokinesThe Journal of Infectious Diseases, 2002
- Mycobacterium avium Invades the Intestinal Mucosa Primarily by Interacting with EnterocytesInfection and Immunity, 2001
- Complete structure of the cell surface polysaccharide of Streptococcus oralis C104: a 600-MHz NMR studyBiochemistry, 1991
- The vitelline envelope to fertilization envelope conversion in eggs of Xenopus laevisDevelopmental Biology, 1986