Complete nucleotide sequence of immunogenic protein MPB70 from Mycobacterium bovis BCG

Abstract

The extracellular protein MPB70 is a heat-stable immunogenic protein which was found in the culture filtrate of Mycobacterium bovis BCG Japanese. We determined the complete nt and aa sequences of MPB70 and correlated with the previously reported data. The N-terminal sequence revealed that the signal peptide (SP) consisted of 30 aa and that the mature protein had 163 aa with a molecular weight of 16 305. The SP displayed a characteristic feature of an Ala-rich property which would be efficient in a SP function.