Catabolic Pathway for the Production of Skatole and Indoleacetic Acid by the Acetogen Clostridium drakei , Clostridium scatologenes , and Swine Manure
- 15 March 2008
- journal article
- Published by American Society for Microbiology in Applied and Environmental Microbiology
- Vol. 74 (6), 1950-1953
- https://doi.org/10.1128/aem.02458-07
Abstract
Skatole (3-methylindole) is a malodorous chemical in stored swine manure and is implicated as a component of foul-tasting pork. Definitive evidence for the skatole pathway is lacking. Deuterium-labeled substrates were employed to resolve this pathway in the acetogenic bacterium Clostridium drakei and Clostridium scatologenes and to determine if a similar pathway is used by microorganisms present in stored swine manure. Indoleacetic acid (IAA) was synthesized from tryptophan by both bacteria, and skatole was synthesized from both IAA and tryptophan. Microorganisms in swine manure produced skatole and other oxidation products from tryptophan, but IAA yielded only skatole. A catabolic mechanism for the synthesis of skatole is proposed.Keywords
This publication has 31 references indexed in Scilit:
- Clostridium carboxidivorans sp. nov., a solvent-producing clostridium isolated from an agricultural settling lagoon, and reclassification of the acetogen Clostridium scatologenes strain SL1 as Clostridium drakei sp. nov.International Journal of Systematic and Evolutionary Microbiology, 2005
- Clostridium scatologenes strain SL1 isolated as an acetogenic bacterium from acidic sediments.International Journal of Systematic and Evolutionary Microbiology, 2000
- Characterization of Volatile Organic Emissions and Wastes from a Swine Production FacilityJournal of Environmental Quality, 1997
- Heterologous expression of an endoglucanase gene (endA) from the ruminai anaerobeRuminococcus flavefaciens17 inStreptococcus bovisandStreptococcus sanguisFEMS Microbiology Letters, 1995
- Physiological aspects of androstenone and skatole formation in the boar—A review with experimental dataMeat Science, 1994
- Metabolism and Bioactivation of 3-Methylindole by Clara Cells, Alveolar Macrophages, and Subcellular Fractions from Rabbit LungsToxicology and Applied Pharmacology, 1993
- Gas chromatographic determination and mass spectrometric confirmation of traces of indole and 3-methylindole (skatole) in pig back fatJournal of Chromatography A, 1985
- Metabolic conversion of 14C-indole-3-acetic acid to 14C-oxindole-3-acetic acidBiochemical and Biophysical Research Communications, 1981
- Origin of objectionable odorous components in piggery wastes and the possibility of applying indicator components for studying odour developmentAgriculture and Environment, 1980
- Mass spectrometry of simple indolesThe Journal of Organic Chemistry, 1968