Abstract
Strains (198) of A. pullulans were screened for their ability to release extracellular hydrolytic enzymes into the cultural medium. Most of the isolates produced, to a varying extent, enzymes such as amylase, lipase (with different fats as substrates), protease (as caseinolysis and gelatin liquefaction), nucleases [RNase, DNase], urease and phosphatase. Neither cellulase nor chitinase were produced. Since some enzymes showed a high level of activity, A. pullulans could possibly be considered a promising source of extracellular lytic enzymes.