Demethylation-Induced Developmental Pleiotropy in Arabidopsis
- 2 August 1996
- journal article
- other
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 273 (5275), 654-657
- https://doi.org/10.1126/science.273.5275.654
Abstract
The function of DNA methylation in higher plants was investigated by expression of a complementary DNA encoding a cytosine methyltransferase (MET1) from Arabidopsis thaliana as an antisense RNA in transgenic plants. This expression resulted in a 34 to 71 percent reduction in total genomic cytosine methylation. Loss of methylation was observed in both repetitive DNA and single-copy gene sequences. Developmental effects included altered heterochrony, changes in meristem identity and organ number, and female sterility. Cytosine demethylation prolonged both vegetative and reproductive phases of development. These findings implicate DNA methylation in establishing or maintaining epigenetic developmental states in the meristem.Keywords
This publication has 29 references indexed in Scilit:
- Gene Trap Tagging of PROLIFERA , an Essential MCM2-3-5 -Like Gene in ArabidopsisScience, 1995
- Arabidopsis thaliana DNA Methylation MutantsScience, 1993
- Genetic Interactions That Regulate Inflorescence Development in Arabidopsis.Plant Cell, 1993
- Genetic Interactions That Regulate Inflorescence Development in ArabidopsisPlant Cell, 1993
- A Phosphoribosylanthranilate Transferase Gene Is Defective in Blue Fluorescent Arabidopsis thaliana Tryptophan MutantsPlant Physiology, 1992
- Targeted mutation of the DNA methyltransferase gene results in embryonic lethalityCell, 1992
- Sequence and organization of 5S ribosomal RNA-encoding genes of Arabidopsis thalianaGene, 1992
- Phase Change and the Regulation of Shoot Morphogenesis in PlantsScience, 1990
- Introduction of a Chimeric Chalcone Synthase Gene into Petunia Results in Reversible Co-Suppression of Homologous Genes in transPlant Cell, 1990
- Cloning and sequencing of a cDNA encoding DNA methyltransferase of mouse cellsJournal of Molecular Biology, 1988