Genetic Analysis of Developmentally Regulated Resistance to Downy Mildew (Hyaloperonospora parasitica) in Arabidopsis thaliana
Open Access
- 1 November 2005
- journal article
- Published by Scientific Societies in Molecular Plant-Microbe Interactions®
- Vol. 18 (11), 1226-1234
- https://doi.org/10.1094/mpmi-18-1226
Abstract
Although developmentally regulated disease resistance has been observed in a variety of plant-pathogen interactions, the molecular basis of this phenomenon is not well understood. Arabidopsis thaliana ecotype Columbia-0 (Col-0) expresses a developmentally regulated resistance to Hyaloperonospora parasitica isolate Emco5. Col-0 seedlings support profuse mycelial growth and asexual spore formation in the cotyledons. In contrast, Emco5 growth and reproducetion is dramatically (but not completely) restricted in the first set of true leaves. Subsequent leaves exhibit progresssively increased resistance. This adult resistance is strongly suppressed by expression of the salicylic acid-degrading transgene NahG and by loss-of-function mutations in the defense-response regulators PAD4, NDR1, RAR1, PBS3 and NPR1. In contrast to Col-0, the Wassilewskija-0 (Ws-0) ecotype supports profuse growth of Emco5 at all stages of development. Gene-dosage experiments and segregation patterns indicate that adult susceptibility in Ws-0 is incomepletely dominant to adult resistance in Col-0. Genetic mapping in a Col × Ws F2 population revealed a major locus on the bottom arm of chromosome 5, which we named RPP31. Analysis of T-DNA insertion lines indicated that the Columbia allele of RPP8, though tightly linked to RPP31, is not necessary for adult resistance.Keywords
This publication has 82 references indexed in Scilit:
- Arabidopsis Downy Mildew Resistance Gene RPP27 Encodes a Receptor-Like Protein Similar to CLAVATA2 and Tomato Cf-9Plant Physiology, 2004
- Developmental Regulation of Mi-Mediated Aphid Resistance Is Independent of Mi-1.2 Transcript LevelsMolecular Plant-Microbe Interactions®, 2004
- Genome-wide analysis of defense-responsive genes in bacterial blight resistance of rice mediated by the recessive R gene xa13Molecular Genetics and Genomics, 2004
- Genome-Wide Insertional Mutagenesis of Arabidopsis thalianaScience, 2003
- Plant pathogens and integrated defence responses to infectionNature, 2001
- THE SPECIFICATION OF LEAF IDENTITY DURING SHOOT DEVELOPMENTAnnual Review of Cell and Developmental Biology, 1998
- The Arabidopsis NPR1 Gene That Controls Systemic Acquired Resistance Encodes a Novel Protein Containing Ankyrin RepeatsCell, 1997
- Effectiveness of Adult-Plant Resistance in Reducing Grain Yield Loss to Powdery Mildew in Winter WheatPlant Disease, 1993
- Screening of fungicides for the control of downy mildew on container-grown cabbage seedlingsCrop Protection, 1992
- Evidence for the occurrence of the “pathogenesis-related” proteins in leaves of healthy tobacco plants during floweringPhysiological Plant Pathology, 1981