Brain tubulin polymerization in the absence of "microtubule-associated proteins".
- 1 December 1976
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences of the United States of America
- Vol. 73 (12), 4397-4399
- https://doi.org/10.1073/pnas.73.12.4397
Abstract
Pure tubulin dimer, purified by phosphecellulose chromatography to remove microtubule associated proteins, can be assembled into microtubules in the presence of dimethyl sulfoxide. The surfaces of such microtubules are devoid of filamentous material. The reaction is rapid and strongly dependent on protein concentration. No microtubule formation occurs from the purified tubulin when dimethyl sulfoxide is omitted.Keywords
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