Real‐time observation of acrosomal dispersal from mouse sperm using GFP as a marker protein
Open Access
- 23 April 1999
- journal article
- Published by Wiley in FEBS Letters
- Vol. 449 (2-3), 277-283
- https://doi.org/10.1016/s0014-5793(99)00433-0
Abstract
We produced transgenic mouse lines that accumulate mutated green fluorescent protein (EGFP) in sperm acrosome, a membrane limited organelle overlying the nucleus. The sperm showed normal fertilizing ...Keywords
This publication has 25 references indexed in Scilit:
- Spermatozoa lacking acrosin protein show delayed fertilizationMolecular Reproduction and Development, 1997
- Green Fluorescent Protein as a Marker for Gene ExpressionScience, 1994
- Acrosome reaction induced by immunoaggregation of a proteinase inhibitor bound to the murine sperm headMolecular Reproduction and Development, 1991
- Aggregation of β-1,4-galactosyltransferase on mouse sperm induces the acrosome reactionDevelopmental Biology, 1991
- Endpoint of first stage of zona pellucida‐induced acrosome reaction in mouse spermatozoa characterized by acrosomal H+ and Ca2+ permeability: Population and single cell kineticsGamete Research, 1989
- The Physiology of Sperm Recovered from the Human Cervix: Acrosomal Status and Response to Inducers of the Acrosome Reaction1Biology of Reproduction, 1989
- Ultrastructural studies of the early events of the human sperm acrosome reaction as initiated by human follicular fluidGamete Research, 1988
- Outer acrosomal membrane of guinea pig spermatozoa: Isolation and structural characterizationGamete Research, 1987
- Determination of the time course of capacitation in mouse spermatozoa using a chlortetracycline fluorescence assayDevelopmental Biology, 1984
- Studies on the fertilization of mouse eggs in vitroThe Japanese journal of animal reproduction, 1971