An anticatalytic monoclonal antibody to avian plasminogen activator: Its effect on behavior of RSV-transformed chick fibroblasts
- 20 June 1986
- journal article
- Published by Elsevier BV in Cell
- Vol. 45 (6), 905-915
- https://doi.org/10.1016/0092-8674(86)90565-9
Abstract
A monoclonal antibody has been raised against the serine protease, plasminogen activator (PA) produced by Rous sarcoma virus-transformed chick embryo fibroblasts (RSVCEF), and selected for its ability to inhibit the catalytic activity of PA. The high specificity and anticatalytic nature of the antibody has allowed probing of the direct role of PA in cellular behavior. Microgram quantities of monoclonal IgG inhibit the overgrowth and the morphological changes associated with RSVCEF transformation and the degradation of extracellular matrix mediated by RSVCEF, indicating a catalytic role for PA in these cellular processes. Specific cleavage of extracellular matrix proteins by immunoaffinity-purified PA in the complete absence of plasminogen demonstrates a direct catalytic involvement of PA in matrix degradation.Keywords
This publication has 42 references indexed in Scilit:
- Cell migrations in embryosCell, 1984
- Expression of transformation-associated protease(s) that degrade fibronectin at cell contact sites.The Journal of cell biology, 1984
- Rat myoblast fusion requires metalloendoprotease activityCell, 1983
- Rat mammary carcinoma cells secrete active collagenase and activate latent enzyme in the stroma via plasminogen activatorInternational Journal of Cancer, 1981
- Analysis of migratory behavior of neural crest and fibroblastic cells in embryonic tissuesDevelopmental Biology, 1980
- Cell surface action of thrombin is sufficient to initiate division of chick cellsCell, 1978
- Immunological characterization of a major transformation-sensitive fibroblast cell surface glycoprotein. Localization, redistribution, and role in cell shape.The Journal of cell biology, 1978
- Isolation of pure IgG1, IgG2a and IgG2b immunoglobulins from mouse serum using protein A-SepharoseImmunochemistry, 1978
- Endogenous Activation of Latent Collagenase by Rheumatoid Synovial CellsNew England Journal of Medicine, 1977
- The preparation of human fibrinogen free of plasminogenBiochimica et Biophysica Acta, 1962