Possible role of IL-2 deficiency for hypogammaglobulinaemia in patients with common variable immunodeficiency
- 1 August 1992
- journal article
- Published by Oxford University Press (OUP) in Clinical and Experimental Immunology
- Vol. 89 (2), 204-210
- https://doi.org/10.1111/j.1365-2249.1992.tb06933.x
Abstract
Common variable immunodeficiency (CVID) patients are unable to produce specific immunoglobulins after antigen contact in vivo. The aim of this study was to investigate whether in some cases of CVID a decreased de novo synthesis of IL-2 might be the cause of immunodeficiency and whether this deficiency can be corrected by IL-2 supplementation in vitro. Mononuclear cells from 17 CVID patients and from 10 healthy controls were cultured with monoclonal anti-CD3 antibody OKT3, pokeweed mitogen (PWM) or tetanus toxoid (TT) to stimulate IL-2 synthesis. In parallel, in vitro IgG and IgM synthesis was stimulated with Staphylococcus aureus Cowan I (SAC), PWM or TT in the presence or absence of IL-2. While lymphocytes of 11 out of 17 patients produced low to normal amounts of IL-2 upon stimulation with anti-CD3, only three patients showed low IL-2 production in response to PWM and five in response to TT. Regarding immunoglobulin synthesis in vitro, five patients completely failed to produce IgM or IgG upon stimulation with PWM, SAC or TT irrespective of the addition of IL-2. By contrast, four patients did not show any defect in vitro and synthesized normal amounts of IgM and IgG with any of the three stimuli. Finally, eight patients could be reconstituted for PWM-, SAC- and TT-induced IgM and/or IgG synthesis in vitro, by adding IL-2 to the culture system. This enhancing effect of IL-2 could be blocked by adding anti-IL-2 receptor antibodies to the cultures. Our findings indicate that a defective IL-2 synthesis after antigen stimulation may be one reason for the impaired immunoglobulin production in some cases of CVID.Keywords
This publication has 25 references indexed in Scilit:
- Abnormalities in CD4+ T lymphocyte subsets in patients with common variable immunodeficiencyClinical Immunology and Immunopathology, 1991
- Elevated serum interleukin-6 associated with a failur in B cell differentiation in common variable immunodeficiecy†Journal of Allergy and Clinical Immunology, 1990
- DEFECTIVE-DNA SYNTHESIS BY T-CELLS IN ACQUIRED COMMON-VARIABLE HYPOGAMMAGLOBULINEMIA ON STIMULATION WITH MITOGENS1989
- Analysis of B cell dysfunction in patients with common variable immunodeficiency by using recombinant interleukin 2.The Tohoku Journal of Experimental Medicine, 1987
- Induction of IgM production by pokeweed mitogen and interleukin-2: Different responses by human peripheral blood cells and tonsil cellsClinical Immunology and Immunopathology, 1986
- T cell receptor triggering induces responsiveness to interleukin 1 and interleukin 2 but does not lead to T cell proliferation.The Journal of Immunology, 1986
- T cell unresponsiveness to the mitogenic activity of OKT3 antibody results from a deficiency of monocyte Fc gamma receptors for murine IgG2a and inability to cross-link the T3-Ti complex.The Journal of Immunology, 1985
- Quantitative measurements of T- and B-cell function in "variable" primary hypogammaglobulinaemia: evidence for a consistent B-cell defect.1977
- The role of suppressor cells in the pathogenesis of common variable hypogammaglobulinemia and the immunodeficiency associated with myeloma.1976
- Accelerated breakdown of immunoglobulin G (IgG) in myotonic dystrophy: a hereditary error of immunoglobulin catabolism.JCI Insight, 1966