Generation of a floxed allele of the mouse BMP type II receptor gene
- 1 March 2005
- Vol. 41 (3), 133-137
- https://doi.org/10.1002/gene.20099
Abstract
Bone morphogenetic proteins (BMPs) regulate a wide range of cellular functions that contribute to embryonic development from mesoderm formation to organogenesis. BMP type II receptor (BMPR-II) transduces BMP signals by forming heteromeric complexes with and phosphorylating BMP type I receptors. Heterozygous germline mutations of BMPR-II gene have been identified in patients with familial and sporadic primary pulmonary hypertension, indicating that BMPR-II may contribute to the maintenance of normal pulmonary vascular structure and function. Since embryos homozygous for a null BMPR-II allele died during gastrulation, precluding further studies of BMPR-II function in organ formation and in adult tissues, we generated mice carrying a conditional mutant BMPR-II allele in which exons 4 and 5 were flanked by loxP sequences. We anticipate that studies of mice carrying a floxed BMPR-II allele and a Cre transgene (under the control of a tissue-specific promoter) will enable characterization of the role of BMPR-II in specific cell types during development and in the pathogenesis of cardiovascular diseases. genesis 41:133–137, 2005.Keywords
This publication has 18 references indexed in Scilit:
- BMPR-IIheterozygous mice have mild pulmonary hypertension and an impaired pulmonary vascular remodeling response to prolonged hypoxiaAmerican Journal of Physiology-Lung Cellular and Molecular Physiology, 2004
- Smad-dependent and Smad-independent pathways in TGF-β family signallingNature, 2003
- BMP signaling is required for septation of the outflow tract of the mammalian heartDevelopment, 2003
- Genetic Analysis of the Mammalian Transforming Growth Factor-β SuperfamilyEndocrine Reviews, 2002
- Divergence and convergence of TGF‐β/BMP signalingJournal of Cellular Physiology, 2001
- TGFβ Signaling in Growth Control, Cancer, and Heritable DisordersCell, 2000
- BMP Type II Receptor Is Required for Gastrulation and Early Development of Mouse EmbryosDevelopmental Biology, 2000
- Signal transduction by bone morphogenetic proteinsCytokine & Growth Factor Reviews, 1998
- Efficient in vivo manipulation of mouse genomic sequences at the zygote stage.Proceedings of the National Academy of Sciences, 1996
- Targeted mutation of the DNA methyltransferase gene results in embryonic lethalityCell, 1992