Anticardiolipin antibodies recognize beta 2-glycoprotein I structure altered by interacting with an oxygen modified solid phase surface.
Open Access
- 1 February 1994
- journal article
- Published by Rockefeller University Press in The Journal of Experimental Medicine
- Vol. 179 (2), 457-462
- https://doi.org/10.1084/jem.179.2.457
Abstract
Anticardiolipin antibodies (aCL) derived from the sera of individuals exhibiting the antiphospholipid syndrome (APS) directly bind to beta 2-glycoprotein I (beta 2-GPI), which is adsorbed to an oxidized polystyrene surface. Oxygen atoms were introduced on a polystyrene surface by irradiation with electron or gamma-ray radiation. X-ray photoelectron spectroscopy revealed the irradiated surfaces were oxidized to generate C-O and C = O moieties. aCL derived from either APS patients or (NZW x BXSB)F1 mice bound to beta 2-GPI coated on the irradiated plates, depending on the radiation dose. Antibody binding to beta 2-GPI on the irradiated plates was competitively inhibited by simultaneous addition of cardiolipin (CL)-coated latex beads mixed together with beta 2-GPI but were unaffected by addition of excess beta 2-GPI, CL micelles, or CL-coated latex beads alone. There was a high correlation between binding values of aCL in sera from 40 APS patients obtained by the anti-beta 2-GPI enzyme-linked immunosorbent assay (ELISA) using the irradiated plates and those by the beta 2-GPI-dependent aCL ELISA. Therefore, aCL have specificity for an epitope on beta 2-GPI. This epitope is expressed by a conformational change occurring when beta 2-GPI interacts with an oxygen-substituted solid phase surface.Keywords
This publication has 23 references indexed in Scilit:
- Molecular definition of human β2-glycoprotein (α2-GPI) by cDNA cloning and inter-species differences of β2-GPI in alternation of anticardiolipin bindingInternational Immunology, 1991
- Anti-phospholipid antibodies are directed against a complex antigen that includes a lipid-binding inhibitor of coagulation: beta 2-glycoprotein I (apolipoprotein H).Proceedings of the National Academy of Sciences of the United States of America, 1990
- Anticardiolipin antibodies—clinical associationsPublished by Oxford University Press (OUP) ,1986
- The anticardiolipin syndrome.1986
- Anti-phospholipid antibodies.1985
- Antibody to Cardiolipin as a Predictor of Fetal Distress or Death in Pregnant Patients with Systemic Lupus ErythematosusNew England Journal of Medicine, 1985
- Anti-phospholipid antibodies and biological false positive serological test for syphilis in patients with systemic lupus erythematosus.1984
- ANTICARDIOLIPIN ANTIBODIES: DETECTION BY RADIOIMMUNOASSAY AND ASSOCIATION WITH THROMBOSIS IN SYSTEMIC LUPUS ERYTHEMATOSUSThe Lancet, 1983
- Surface modification and evaluation of some commonly used catheter materials. I. Surface propertiesJournal of Biomedical Materials Research, 1983
- Phosphorus Assay in Column ChromatographyJournal of Biological Chemistry, 1959