The apolipoprotein B mRNA editing complex performs a multifunctional cycle and suppresses nonsense-mediated decay
- 1 August 2003
- journal article
- Published by Springer Science and Business Media LLC in The EMBO Journal
- Vol. 22 (15), 3971-3982
- https://doi.org/10.1093/emboj/cdg369
Abstract
The C to U editing of apolipoprotein B (apoB) mRNA is mediated by a minimal complex composed of an RNA‐binding cytidine deaminase (APOBEC1) and a complementing specificity factor (ACF). This editing generates a premature termination codon and a truncated open reading frame. We demonstrate that the APOBEC1—ACF holoenzyme mediates a multifunctional cycle. The atypical APOBEC1 nuclear localization signal is involved in RNA binding and is used to import ACF into the nucleus as cargo. APOBEC1 alone induces nonsense‐mediated decay (NMD). The APOBEC1—ACF complex edits and remains associated with the edited RNA to protect it from NMD. The APOBEC1 nuclear export signal is involved in the export of ACF and the edited apoB mRNA together, to the site of translation.Keywords
This publication has 51 references indexed in Scilit:
- A novel translational repressor mRNA is edited extensively in livers containing tumors caused by the transgene expression of the apoB mRNA-editing enzyme.Genes & Development, 1997
- Leptomycin B is an inhibitor of nuclear export: inhibition of nucleo-cytoplasmic translocation of the human immunodeficiency virus type 1 (HIV-1) Rev protein and Rev-dependent mRNACell Chemical Biology, 1997
- Nucleocytoplasmic TransportScience, 1996
- The HIV-1 Rev Activation Domain is a nuclear export signal that accesses an export pathway used by specific cellular RNAsCell, 1995
- A place in the world for RNA editingCell, 1995
- Nuclear localization signals overlap DNA- or RNA-binding domains in nucleic acid-binding proteinsNucleic Acids Research, 1995
- Evolutionary origins of apoB mRNA editing: Catalysis by a cytidine deaminase that has acquired a novel RNA-binding motif at its active siteCell, 1995
- A yeast RNA-binding protein shuttles between the nucleus and the cytoplasm.Molecular and Cellular Biology, 1994
- The p27 catalytic subunit of the apolipoprotein B mRNA editing enzyme is a cytidine deaminase.Journal of Biological Chemistry, 1993
- Apolipoprotein B mRNA editing is associated with UV crosslinking of proteins to the editing site.Proceedings of the National Academy of Sciences of the United States of America, 1993