The genus Rualena Chamberlin & Ivie 1942 (Araneae: Agelenidae) is revised and a new genus, Hoffmannilena, is described. Four new species are described for Rualena: R. cedros sp. nov. (♂ ♀), R. parritas sp. nov. (♂ ♀), R. thomas sp. nov. (♂ ♀), and R. ubicki sp. nov. (♀). The males of R. alleni Chamberlin & Ivie 1942 and R. avila Chamberlin & Ivie 1942 and the female of R. rua (Chamberlin 1919) are described for the first time. Rualena goleta Chamberlin & Ivie 1942 is a junior synonym of R. surana Chamberlin & Ivie 1942. Rualena cavata (F.O. Pickard-Cambridge 1902) and R. pasquinii Brignoli 1974 are considered incertae sedis. Two species are transferred from Rualena to Novalena Chamberlin & Ivie 1942: N. shlomitae (García-Villafuerte 2009) comb. nov. and N. simplex (F.O. Pickard-Cambridge 1902) comb. nov. For Hoffmannilena gen. nov., five new species are described: H. apoala sp. nov. (♀), H. cumbre sp. nov. (♂ ♀), H. huajuapan sp. nov. (♀), H. mitla sp. nov. (♀), and H. tizayuca sp. nov. (♂ ♀). Three species are transferred from Novalena to Hoffmannilena gen. nov.: H. lobata (F.O. Pickard-Cambridge 1902) comb. nov., H. marginata (F.O. Pickard-Cambridge 1902) comb. nov., and H. variabilis (F.O. Pickard-Cambridge 1902) comb. nov. Novalena cuspidata (F.O. Pickard-Cambridge 1902) is a junior synonym of H. marginata.