Proper Recruitment of γ-Tubulin and D-TACC/Msps to EmbryonicDrosophilaCentrosomes Requires Centrosomin Motif 1
- 1 October 2007
- journal article
- Published by American Society for Cell Biology (ASCB) in Molecular Biology of the Cell
- Vol. 18 (10), 4037-4049
- https://doi.org/10.1091/mbc.e07-05-0474
Abstract
Centrosomes are microtubule-organizing centers and play a dominant role in assembly of the microtubule spindle apparatus at mitosis. Although the individual binding steps in centrosome maturation are largely unknown, Centrosomin (Cnn) is an essential mitotic centrosome component required for assembly of all other known pericentriolar matrix (PCM) proteins to achieve microtubule-organizing activity at mitosis in Drosophila. We have identified a conserved motif (Motif 1) near the amino terminus of Cnn that is essential for its function in vivo. Cnn Motif 1 is necessary for proper recruitment of γ-tubulin, D-TACC (the homolog of vertebrate transforming acidic coiled-coil proteins [TACC]), and Minispindles (Msps) to embryonic centrosomes but is not required for assembly of other centrosome components including Aurora A kinase and CP60. Centrosome separation and centrosomal satellite formation are severely disrupted in Cnn Motif 1 mutant embryos. However, actin organization into pseudocleavage furrows, though aberrant, remains partially intact. These data show that Motif 1 is necessary for some but not all of the activities conferred on centrosome function by intact Cnn.Keywords
This publication has 56 references indexed in Scilit:
- Making Microtubules and Mitotic Spindles in Cells without Functional CentrosomesCurrent Biology, 2006
- Aurora A activates D-TACC–Msps complexes exclusively at centrosomes to stabilize centrosomal microtubulesThe Journal of cell biology, 2005
- Function and regulation of Maskin, a TACC family protein, in microtubule growth during mitosisThe Journal of cell biology, 2005
- Aurora A phosphorylation of TACC3/maskin is required for centrosome-dependent microtubule assembly in mitosisThe Journal of cell biology, 2005
- Efficient formation of bipolar microtubule bundles requires microtubule-bound γ-tubulin complexesThe Journal of cell biology, 2005
- A centrosomal mechanism involving CDK5RAP2 and CENPJ controls brain sizeNature Genetics, 2005
- Drosophila Nek2 protein kinase knockdown leads to centrosome maturation defects while overexpression causes centrosome fragmentation and cytokinesis failureExperimental Cell Research, 2004
- Interaction of Aurora-A and centrosomin at the microtubule-nucleating site in Drosophila and mammalian cellsThe Journal of cell biology, 2003
- Organized microtubule arrays in γ-tubulin-depleted Drosophila spermatocytesCurrent Biology, 2001
- Developmental defects of female-sterile mutants of Drosophila melanogasterDevelopmental Biology, 1975