Chicken Juice Enhances Surface Attachment and Biofilm Formation of Campylobacter jejuni
Open Access
- 15 November 2014
- journal article
- Published by American Society for Microbiology in Applied and Environmental Microbiology
- Vol. 80 (22), 7053-7060
- https://doi.org/10.1128/aem.02614-14
Abstract
The bacterial pathogen Campylobacter jejuni is primarily transmitted via the consumption of contaminated foodstuffs, especially poultry meat. In food processing environments, C. jejuni is required to survive a multitude of stresses and requires the use of specific survival mechanisms, such as biofilms. An initial step in biofilm formation is bacterial attachment to a surface. Here, we investigated the effects of a chicken meat exudate (chicken juice) on C. jejuni surface attachment and biofilm formation. Supplementation of brucella broth with ≥5% chicken juice resulted in increased biofilm formation on glass, polystyrene, and stainless steel surfaces with four C. jejuni isolates and one C. coli isolate in both microaerobic and aerobic conditions. When incubated with chicken juice, C. jejuni was both able to grow and form biofilms in static cultures in aerobic conditions. Electron microscopy showed that C. jejuni cells were associated with chicken juice particulates attached to the abiotic surface rather than the surface itself. This suggests that chicken juice contributes to C. jejuni biofilm formation by covering and conditioning the abiotic surface and is a source of nutrients. Chicken juice was able to complement the reduction in biofilm formation of an aflagellated mutant of C. jejuni , indicating that chicken juice may support food chain transmission of isolates with lowered motility. We provide here a useful model for studying the interaction of C. jejuni biofilms in food chain-relevant conditions and also show a possible mechanism for C. jejuni cell attachment and biofilm initiation on abiotic surfaces within the food chain.Keywords
This publication has 46 references indexed in Scilit:
- The Effect of a Silver Nanoparticle Polysaccharide System on Streptococcal and Saliva-Derived BiofilmsInternational Journal of Molecular Sciences, 2013
- Characterization of Mono- and Mixed-Culture Campylobacter jejuni BiofilmsApplied and Environmental Microbiology, 2012
- Influence of Season and Geography on Campylobacter jejuni and C. coli Subtypes in Housed Broiler Flocks Reared in Great BritainApplied and Environmental Microbiology, 2011
- Bacteriophage-Mediated Dispersal of Campylobacter jejuni BiofilmsApplied and Environmental Microbiology, 2011
- Enhanced Surface Colonization by Escherichia coli O157:H7 in Biofilms Formed by an Acinetobacter calcoaceticus Isolate from Meat-Processing EnvironmentsApplied and Environmental Microbiology, 2010
- Biofilm Formation by Campylobacter jejuni Is Increased under Aerobic ConditionsApplied and Environmental Microbiology, 2010
- Staphylococcus aureus -Fibronectin Interactions with and without Fibronectin-Binding Proteins and Their Role in Adhesion and DesorptionApplied and Environmental Microbiology, 2008
- The Complete Genome Sequence of Campylobacter jejuni Strain 81116 (NCTC11828)Journal of Bacteriology, 2007
- Characterization of Campylobacter jejuni Biofilms under Defined Growth ConditionsApplied and Environmental Microbiology, 2007
- Unique Features of a Highly Pathogenic Campylobacter jejuni StrainInfection and Immunity, 2006