Oscillatory Expression of the bHLH Factor Hes1 Regulated by a Negative Feedback Loop
- 25 October 2002
- journal article
- other
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 298 (5594), 840-843
- https://doi.org/10.1126/science.1074560
Abstract
Transcription of messenger RNAs (mRNAs) for Notch signaling molecules oscillates with 2-hour cycles, and this oscillation is important for coordinated somite segmentation. However, the molecular mechanism of such oscillation remains to be determined. Here, we show that serum treatment of cultured cells induces cyclic expression of both mRNA and protein of the Notch effector Hes1, a basic helix-loop-helix (bHLH) factor, with 2-hour periodicity. Cycling is cell-autonomous and depends on negative autoregulation of hes1 transcription and ubiquitin-proteasome–mediated degradation of Hes1 protein. Because Hes1 oscillation can be seen in many cell types, this clock may regulate timing in many biological systems.Keywords
This publication has 26 references indexed in Scilit:
- Clock Regulatory Elements Control Cyclic Expression of Lunatic fringe during SomitogenesisDevelopmental Cell, 2002
- Periodic Lunatic fringe Expression Is Controlled during Segmentation by a Cyclic Transcriptional Enhancer Responsive to Notch SignalingDevelopmental Cell, 2002
- Vertebrate SomitogenesisAnnual Review of Cell and Developmental Biology, 2001
- Molecular Targets of Vertebrate Segmentation: Two Mechanisms Control Segmental Expression of Xenopus hairy2 during Somite FormationDevelopmental Cell, 2001
- Dynamic expression and essential functions of Hes7 in somite segmentationGenes & Development, 2001
- Molecular Analysis of Mammalian Circadian RhythmsAnnual Review of Physiology, 2001
- THE UBIQUITIN SYSTEMAnnual Review of Biochemistry, 1998
- Mediation of NGF signaling by post-translational inhibition of HES-1, a basic helix–loop–helix repressor of neuronal differentiationPublished by Cold Spring Harbor Laboratory ,1997
- Signalling downstream of activated mammalian NotchNature, 1995
- Stimulus-Transcription Coupling in the Nervous System: Involvement of the Inducible Proto-Oncogenes fos and junAnnual Review of Neuroscience, 1991