Detection of a soluble acrosome reaction‐inducing factor, different from serum albumin, associated with the ovulated egg‐cumulus complex
- 1 December 1991
- journal article
- Published by Wiley in Molecular Reproduction and Development
- Vol. 30 (4), 396-401
- https://doi.org/10.1002/mrd.1080300415
Abstract
Soluble extracts of the ovulated hamster egg‐cumulus complex (ECC) were tested on capacitated sperm for activity in inducing the physiological acrosome reaction (AR). Evidence for occurrence of the physiological AR included enhanced sperm penetration of intact homologous zonae pellucidae as well as induction of AR in nonattached and in zona‐bound sperm following a brief coincubation with test compound. Since hamster serum albumin, a major protein of hamster body fluids, also induces spontaneous ARs under certain conditions, it was used as one of the comparators for the acrosome reaction inducing factor (ARIF; Westrick et al., Biol Reprod 32 [Suppl 1]. 213, 1985) activity in the ECC. Sperm exposure to concentrations of the soluble ECC extract ranging from 0.04 to 0.2 mg protein/ml significantly increased penetration of salt‐stored zonae by 36%, mean numbers of penetrating sperm by 90%, ARs in nonattached sperm by 65%, and ARs in zona‐bound sperm by 102%. Hamster serum albumin added after completion of capacitation had no significant effect on these parameters. We conclude that (1) the ovulated ECC contains a soluble ARIF that augments zona‐induced ARs and sperm penetration and (2) the ARIF is not serum albumin.Keywords
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