Characterization of the pathogenicity island protein PdpA and its role in the virulence of Francisella novicida
- 1 May 2009
- journal article
- Published by Microbiology Society in Microbiology
- Vol. 155 (5), 1489-1497
- https://doi.org/10.1099/mic.0.025379-0
Abstract
Francisella tularensisis a highly virulent, intracellular pathogen that causes the disease tularaemia. A research surrogate forF. tularensisisFrancisella novicida, which causes a tularaemia-like disease in mice, grows similarly in macrophages, and yet is unable to cause disease in humans. BothFrancisellaspecies contain a cluster of genes referred to as theFrancisellapathogenicity island (FPI). Pathogenicity determinant protein A (PdpA), encoded by thepdpAgene, is located within the FPI and has been associated with the virulence ofFrancisellaspecies. In this work we examined the properties of PdpA protein expression and localization as well as the phenotype of aF. novicida pdpAdeletion mutant. Monoclonal antibody detection of PdpA showed that it is a soluble protein that is upregulated in iron-limiting conditions and undetectable in anmglAormglBmutant background. Deletion ofpdpAresulted in a strain that was highly attenuated for virulence in chicken embryos and mice.Keywords
This publication has 34 references indexed in Scilit:
- A Francisella novicida pdpA mutant exhibits limited intracellular replication and remains associated with the lysosomal marker LAMP-1Microbiology, 2009
- Identification of fevR , a Novel Regulator of Virulence Gene Expression in Francisella novicidaInfection and Immunity, 2008
- TheFrancisellaPathogenicity Island Protein PdpD Is Required for Full Virulence and Associates with Homologues of the Type VI Secretion SystemJournal of Bacteriology, 2008
- Secretome Analysis Uncovers an Hcp-Family Protein Secreted via a Type VI Secretion System inAgrobacterium tumefaciensJournal of Bacteriology, 2008
- In vivo negative selection screen identifies genes required for Francisella virulenceProceedings of the National Academy of Sciences, 2007
- Identification of MglA-Regulated Genes Reveals Novel Virulence Factors in Francisella tularensisInfection and Immunity, 2006
- Ralstonia solanacearum requires F-box-like domain-containing type III effectors to promote disease on several host plantsProceedings of the National Academy of Sciences, 2006
- AttenuatedFrancisella novicidaTransposon Mutants Protect Mice against Wild-Type ChallengeInfection and Immunity, 2006
- Virulence ofFrancisellaspp. in Chicken EmbryosInfection and Immunity, 2006
- Identification of Francisella tularensis Genes Affected by Iron LimitationInfection and Immunity, 2006