Surface-modified hyaluronic acid hydrogels to capture endothelial progenitor cells
- 23 August 2010
- journal article
- research article
- Published by Royal Society of Chemistry (RSC) in Soft Matter
- Vol. 6 (20), 5120-5126
- https://doi.org/10.1039/c0sm00508h
Abstract
A major challenge to the effective treatment of injured cardiovascular tissues is the promotion of endothelialization of damaged tissues and implanted devices. For this reason, there is a need for new biomaterials that promote endothelialization to enhance vascular repair. The goal of this work was to develop antibody-modified polysaccharide-based hydrogels that could selectively capture endothelial progenitor cells (EPCs). We showed that CD34 antibody immobilization on hyaluronic acid (HA) hydrogels provides a suitable surface to capture EPCs. The effect of CD34 antibody immobilization on EPC adhesion was found to be dependent on antibody concentration. The highest level of EPC attachment was found to be 52.2 cells per mm2 on 1% HA gels modified with 25 μg mL−1 antibody concentration. Macrophages did not exhibit significant attachment on these modified hydrogel surfaces compared to the EPCs, demonstrating the selectivity of the system. Hydrogels containing only HA, with or without immobilized CD34, did not allow for spreading of EPCs 48 h after cell seeding, even though the cells were adhered to the hydrogel surface. To promote spreading of EPCs, 2% (w/v) gelatin methacrylate (GelMA) containing HA hydrogels were synthesized and shown to improve cell spreading and elongation. This strategy could potentially be useful to enhance the biocompatibility of implants such as artificial heart valves or in other tissue engineering applications where formation of vascular structures is required.Keywords
This publication has 34 references indexed in Scilit:
- Development of microfluidics as endothelial progenitor cell capture technology for cardiovascular tissue engineering and diagnostic medicineThe FASEB Journal, 2009
- New strategies for in vivo tissue engineering by mimicry of homing factors for self-endothelialisation of blood contacting materialsBiomaterials, 2008
- Biology of bone marrow-derived endothelial cell precursorsAmerican Journal of Physiology-Heart and Circulatory Physiology, 2007
- Receptor transactivation cascades.Focus on “Effects of α1D-adrenergic receptors on shedding of biologically active EGF in freshly isolated lacrimal gland epithelial cells”American Journal of Physiology-Cell Physiology, 2007
- Micromolding of photocrosslinkable hyaluronic acid for cell encapsulation and entrapmentJournal of Biomedical Materials Research Part A, 2006
- Effects of cross-linking molecular weights in a hyaluronic acid–poly(ethylene oxide) hydrogel network on its propertiesBiomedical Materials, 2006
- Endothelial progenitor cells: diagnostic and therapeutic considerationsBioEssays, 2006
- Hyaluronic acid hydrogel as Nogo-66 receptor antibody delivery system for the repairing of injured rat brain: in vitroJournal of Controlled Release, 2004
- Designing scaffolds for valvular interstitial cells: Cell adhesion and function on naturally derived materialsJournal of Biomedical Materials Research Part A, 2004
- Morphologic recognition of complex carbohydrates in embryonic cardiac extracellular matrix.Journal of Histochemistry & Cytochemistry, 1979