Food Deprivation Exacerbates Mitochondrial Oxidative Stress in Rat Liver Exposed to Ischemia-Reperfusion Injury
Open Access
- 1 January 2001
- journal article
- research article
- Published by Oxford University Press (OUP) in Journal of Nutrition
- Vol. 131 (1), 105-110
- https://doi.org/10.1093/jn/131.1.105
Abstract
Mitochondria undergo oxidative damage during reperfusion of ischemic liver. Although nutritional status affects ischemia-reperfusion injury in the liver, its effect on mitochondrial damage has not been evaluated. Thus, this study was designed to determine whether starvation influences the oxidative balance in mitochondria isolated from livers exposed to warm ischemia-reperfusion. Fed and 18- and 36-h food-deprived rats underwent partial hepatic ischemia followed by reperfusion. Mitochondria were isolated before and after ischemia and during reperfusion. Serum alanine transaminase was measured to assess liver injury. The mitochondrial concentrations of malondialdehyde, protein carbonyls and glutathione were determined as indicators of oxidative injury. Cell ultrastructure was assessed by transmission electron microscopy. Transaminase levels were greater in 18-h food-deprived than fed rats (after 120 min of reperfusion: 3872 ± 400 vs. 1138 ± 59 U/L, P < 0.01). Mitochondrial glutathione was lower in food-deprived than fed rats before and after ischemia, and during reperfusion. Food deprivation also was associated with significantly greater lipid and protein oxidative damage. Finally, more ultrastructural damage was observed during reperfusion in mitochondria from food-deprived rats. Prolonging the length of food deprivation to 36 h exacerbated significantly both the mitochondrial oxidative injury and the release of serum transaminases in rats (after 120 min of reperfusion: 5438 ± 504 U/L, P < 0.01). Food deprivation was associated with greater mitochondrial oxidative injury in rat livers exposed to warm ischemia-reperfusion, and the extent of oxidative damage in mitochondria increased with the length of food deprivation.Keywords
This publication has 27 references indexed in Scilit:
- Oxidation of circulating proteins in alcoholics: role of acetaldehyde and xanthine oxidaseJournal of Hepatology, 1996
- Mitochondrial dysfunction in ischaemia‐reperfusionActa Anaesthesiologica Scandinavica, 1995
- PROTECTION BY PENTOXIFYLLINE AGAINST NORMOTHERMIC LIVER ISCHEMIA/REPERFUSION IN RATSTransplantation, 1995
- Decreased antioxidant status and increased lipid peroxidation in patients with septic shock and secondary organ dysfunctionCritical Care Medicine, 1995
- ENTERAL FEEDING MINIMIZES LIVER INJURY DURING HEMORRHAGIC SHOCKShock, 1994
- OXIDATION OF FREE AMINO ACIDS AND AMINO ACID RESIDUES IN PROTEINS BY RADIOLYSIS AND BY METAL-CATALYZED REACTIONSAnnual Review of Biochemistry, 1993
- THE BENEFICIAL EFFECT OF CYCLOSPORINE ON LIVER ISCHEMIA IN RATSTransplantation, 1989
- Mitochondrial damage in muscle occurs after marked depletion of glutathione and is prevented by giving glutathione monoester.Proceedings of the National Academy of Sciences of the United States of America, 1989
- Generation of H2O2 in biomembranesBiochimica et Biophysica Acta (BBA) - Reviews on Biomembranes, 1982
- The influence of fasting on the susceptibility of mice to hepatotoxic injuryToxicology and Applied Pharmacology, 1981