The Escherichia coli Regulator of Sigma 70 Protein, Rsd, Can Up-Regulate Some Stress-Dependent Promoters by Sequestering Sigma 70
Open Access
- 1 May 2007
- journal article
- research article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 189 (9), 3489-3495
- https://doi.org/10.1128/jb.00019-07
Abstract
The Escherichia coli Rsd protein forms complexes with the RNA polymerase σ 70 factor, but its biological role is not understood. Transcriptome analysis shows that overexpression of Rsd causes increased expression from some promoters whose expression depends on the alternative σ 38 factor, and this was confirmed by experiments with lac fusions at selected promoters. The LP18 substitution in Rsd increases the Rsd-dependent stimulation of these promoter- lac fusions. Analysis with a bacterial two-hybrid system shows that the LP18 substitution in Rsd increases its interaction with σ 70 . Our experiments support a model in which the role of Rsd is primarily to sequester σ 70 , thereby increasing the levels of RNA polymerase containing the alternative σ 38 factor.Keywords
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