Identification of active compounds and their metabolites by high‐performance liquid chromatography/electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry from Xiao‐xu‐ming decoction (XXMD)
- 28 July 2009
- journal article
- research article
- Published by Wiley in Rapid Communications in Mass Spectrometry
- Vol. 23 (17), 2724-2732
- https://doi.org/10.1002/rcm.4179
Abstract
Xiao‐xu‐ming decoction (XXMD) prescription is a traditional Chinese prescription that has been widely used to treat theoplegia and the sequela of theoplegia. Modern pharmacological research has also indicated that the active fraction from XXMD is able to treat cardiovascular diseases and Alzheimer's disease. In the study reported here, high‐performance liquid chromatography coupled with Fourier transform ion cyclotron resonance mass spectrometry (HPLC/FTICR‐MS) was developed to identify active compounds and their metabolites after oral administration of active fraction from Xiao‐xu‐ming decoction to rats, using parent mass list triggered data‐dependent multiple‐stage mass analysis at a resolving power of 100 000 in the external calibration mode. The mass accuracies obtained for full‐scan MS were within 2 ppm in most cases. Fifteen constituents were identified in the active fraction from XXMD and the biological samples of rats. The fragmentation behaviors of these constituents were summarized which would be helpful for structural characterization. The profiles of the constituents in the active fraction and biological samples of rats were obtained which provided us with much information for a better understanding of the chemical basis of the pharmacologic actions of XXMD. Copyright © 2009 John Wiley & Sons, Ltd.Keywords
Funding Information
- National Nature Science Foundation (30630073)
This publication has 23 references indexed in Scilit:
- Identification and determination of the major constituents in Traditional Chinese Medicinal formula Danggui-Shaoyao-San by HPLC–DAD–ESI-MS/MSJournal of Pharmaceutical and Biomedical Analysis, 2009
- Characterization of fifty‐one flavonoids in a Chinese herbal prescription Longdan Xiegan Decoction by high‐performance liquid chromatography coupled to electrospray ionization tandem mass spectrometry and photodiode array detectionRapid Communications in Mass Spectrometry, 2008
- Towards high-throughput metabolomics using ultrahigh-field Fourier transform ion cyclotron resonance mass spectrometryMetabolomics, 2008
- Profiling of N‐acyl‐homoserine lactones by liquid chromatography coupled with electrospray ionization and a hybrid quadrupole linear ion‐trap and Fourier‐transform ion‐cyclotron‐resonance mass spectrometry (LC‐ESI‐LTQ‐FTICR‐MS)Journal of Mass Spectrometry, 2007
- High-throughput screening assay for groups of effective components extracted from Xiaoxuming RecipeJournal of Chinese Integrative Medicine, 2006
- Accurate mass measurements by Fourier transform mass spectrometryMass Spectrometry Reviews, 2004
- Studies on the flavones using liquid chromatography–electrospray ionization tandem mass spectrometryJournal of Chromatography A, 2004
- Nontargeted Metabolome Analysis by Use of Fourier Transform Ion Cyclotron Mass SpectrometryOMICS: A Journal of Integrative Biology, 2002
- The use of electrospray ionization with Fourier transform ion cyclotron resonance mass spectrometry in the analysis of trace impurities in a drug substanceRapid Communications in Mass Spectrometry, 1995
- The Structure of Cimifugin, a New Bitter Principle from Cimicifuga simplex WORMSK.CHEMICAL & PHARMACEUTICAL BULLETIN, 1972