The Magnaporthe oryzae Avirulence Gene AvrPiz-t Encodes a Predicted Secreted Protein That Triggers the Immunity in Rice Mediated by the Blast Resistance Gene Piz-t
Open Access
- 1 April 2009
- journal article
- research article
- Published by Scientific Societies in Molecular Plant-Microbe Interactions®
- Vol. 22 (4), 411-420
- https://doi.org/10.1094/mpmi-22-4-0411
Abstract
The Magnaporthe oryzae avirulence gene AvrPiz-t activates immunity in a gene-for-gene fashion to rice mediated by the blast resistance gene Piz-t. To dissect the molecular mechanism underlying their recognition, we initiated the cloning of AvrPiz-t using a map-based cloning strategy. The AvrPiz-t gene was delimited to an approximately 21-kb genomic fragment, in which six genes were predicted. Complementation tests of each of these six candidate genes led to the final identification of AvrPiz-t, which encodes a 108-amino-acid predicted secreted protein with unknown function and no homologues in M. oryzae or in other sequenced fungi. We found that AvrPiz-t is present in the virulent isolate GUY11 but contains a Pot3 insertion at a position 462 bp upstream from the start codon. Complementation tests of AvrPiz-t genes driven by promoters of varying length revealed that a promoter larger than 462 bp is essential to maintain the AvrPiz-t function. These results suggest that a Pot3 insertion in GUY11 might interfere with the proper function of AvrPiz-t. Additionally, we found that AvrPiz-t can suppress the programmed cell death triggered by mouse BAX protein in Nicotiana benthamiana, identifying a mechanism by which AvrPiz-t may contribute virulence of M. oryzae.Keywords
This publication has 60 references indexed in Scilit:
- Genome Organization and Evolution of theAVR-PitaAvirulence Gene Family in theMagnaporthe griseaSpecies ComplexMolecular Plant-Microbe Interactions®, 2008
- Conserved C-Terminal Motifs Required for Avirulence and Suppression of Cell Death by Phytophthora sojae effector Avr1bPlant Cell, 2008
- The plant immune systemNature, 2006
- The Eight Amino-Acid Differences Within Three Leucine-Rich Repeats Between Pi2 and Piz-t Resistance Proteins Determine the Resistance Specificity to Magnaporthe griseaMolecular Plant-Microbe Interactions®, 2006
- The map-based sequence of the rice genomeNature, 2005
- An ancestral oomycete locus contains late blight avirulence gene Avr3a , encoding a protein that is recognized in the host cytoplasmProceedings of the National Academy of Sciences of the United States of America, 2005
- The genome sequence of the rice blast fungus Magnaporthe griseaNature, 2005
- Calcium-dependent protein kinases play an essential role in a plant defence responseThe EMBO Journal, 2001
- Plant pathogens and integrated defence responses to infectionNature, 2001
- Current Status of the Gene-For-Gene ConceptAnnual Review of Phytopathology, 1971