Changes in susceptibility of grape berries to splitting are related to impaired osmotic water uptake associated with losses in cell vitality
- 28 September 2010
- journal article
- Published by Hindawi Limited in Australian Journal of Grape and Wine Research
- Vol. 16 (3), 469-476
- https://doi.org/10.1111/j.1755-0238.2010.00108.x
Abstract
Background and Aims: High rainfall events after the onset of ripening commonly cause the skin of grape berries to split. The aim was to verify the suggestion, based on vineyard observations, that the susceptibility of grapes to splitting decreases during ripening, and to establish the role of cell vitality in this process. Methods and Results: The susceptibility of detached, ripening berries, cv. Shiraz, to splitting was assessed by immersing them in sucrose solutions or deionised water. At the onset of ripening, susceptibility to splitting was highest and decreased only slightly over 30 days. Thereafter, susceptibility decreased greatly until becoming negligible about 30 days later. Cell vitality within the pericarp, as determined by nitroblue tetrazolium staining, decreased from the onset of ripening. Conclusions: The reduction in the susceptibility of grape berries to splitting is attributed to a decrease in turgor‐generating capacity within the berry as an increasing proportion of pericarp cells lose vitality. The loss of cell vitality corroborates previous evidence of this phenomenon, and indicates that this is a general feature of grape ripening. Significance of the Study: This study demonstrates the proportion of vital cells within the pericarp is the driver of turgor‐induced splitting, in contrast to previous models in which the whole berry, enclosed in a semi‐permeable skin, has been regarded as the osmotically functional system.Keywords
This publication has 16 references indexed in Scilit:
- Evidence for substantial maintenance of membrane integrity and cell viability in normally developing grape (Vitis vinifera L.) berries throughout developmentJournal of Experimental Botany, 2008
- Morphology, anatomy and development of the pericarp after anthesis in grape, Vitis vinifera L.Australian Journal of Grape and Wine Research, 1996
- Partitioning Control by Water Potential Gradient: Evidence for Compartmentation Breakdown in Grape BerriesJournal of Experimental Botany, 1991
- Susceptibility to Berry Splitting in Several Grape CultivarsJournal of the Japanese Society for Horticultural Science, 1985
- Solute accumulation by grape pericarp cells II. Studies with protoplasts and isolated vacuolesBiochemie und Physiologie der Pflanzen, 1984
- Physical Aspects of Fruit Growth: Cuticular Fracture and Fracture Patterns in Relation to Fruit Structure inVitis ViniferaJournal of Horticultural Science, 1982
- Tetrazolium Salts and FormazansProgress in Histochemistry and Cytochemistry, 1976
- Fruit splitting in grapes: Determination of the critical turgor pressureAustralian Journal of Agricultural Research, 1972
- THE CYTOCHEMICAL REDUCTION OF NITRO BLUE TETRAZOLIUM AS AN INDEX OF POLLEN VIABILITYAmerican Journal of Botany, 1964
- Chemical and sensory variability in table grapesHilgardia, 1963