Failure of a discontinuous bend to prevent lens epithelial cell migration in vitro
- 1 July 2002
- journal article
- Published by Ovid Technologies (Wolters Kluwer Health) in Journal of Cataract & Refractive Surgery
- Vol. 28 (7), 1256-1261
- https://doi.org/10.1016/s0886-3350(02)01209-9
Abstract
Purpose: To assess the effect of substrate geometry (discontinuous bend) on lens epithelial cell (LEC) growth in vitro. Setting: Department of Ophthalmology, St. Thomas’ Hospital, London, United Kingdom. Methods: Culture wells with central depths of 0.4 mm, 1.0 mm, or 3.0 mm and a sharp square-edged profile (discontinuous bend) or a round-edged profile (continuous bend) were produced from a block of poly(methyl methacrylate). Freshly harvested bovine LECs were attached to the center of each well and cultured using standard techniques. Observations were made of whether LECs grew out of the wells and of the time required to do so. Results: Lens epithelial cells migrated out of all the wells. There was no significant difference in the rate at which they migrated out of round-edged and square-edged wells. Conclusions: In vitro, a sharp discontinuous bend did not appear to induce contact inhibition of migrating LECs nor did it significantly hinder the rate at which LECs migrated. Therefore, a discontinuous bend in the lens capsule in isolation is unlikely to be responsible for the observed reduction in posterior capsule opacification associated with the use of square-edged IOLs.Keywords
This publication has 20 references indexed in Scilit:
- Effect of surgical technique on in vitro posterior capsule opacificationJournal of Cataract & Refractive Surgery, 2000
- Double-masked prospective ocular safety study of a lens epithelial cell antibody to prevent posterior capsule opacificationJournal of Cataract & Refractive Surgery, 2000
- A systematic overview of the incidence of posterior capsule opacificationOphthalmology, 1998
- Inhibition of migrating lens epithelial cells by blocking the adhesion molecule integrin: A preliminary reportJournal of Cataract & Refractive Surgery, 1997
- Diclofenac sodium and cyclosporin A inhibit human lens epithelial cell proliferation in cultureAlbrecht von Graefes Archiv für Ophthalmologie, 1997
- Effect of intraocular sustained release of indomethacin on postoperative inflammation and posterior capsule opacificationJournal of Cataract & Refractive Surgery, 1996
- Effect of indomethacin-coated posterior chamber intraocular lenses on postoperative inflammation and posterior capsule opacificationJournal of Cataract & Refractive Surgery, 1995
- Effects of diclofenac sodium and indomethacin on proliferation and collagen synthesis of lens epithelial cells in vitroJournal of Cataract & Refractive Surgery, 1995
- Intercapsular cataract surgery with lens epithelial cell removal Part III: Long-term follow-up of posterior capsular opacificationJournal of Cataract & Refractive Surgery, 1991
- The human anterior lens capsule--an attempted chemical debridement of epithelial cells by ethylenediaminetetracetic acid (EDTA) and trypsin.British Journal of Ophthalmology, 1988