Interplay of impermeability and chromosomal beta-lactamase activity in imipenem-resistant Pseudomonas aeruginosa
- 1 September 1992
- journal article
- Published by American Society for Microbiology in Antimicrobial Agents and Chemotherapy
- Vol. 36 (9), 2046-2048
- https://doi.org/10.1128/aac.36.9.2046
Abstract
Mutational loss of the D2 porin causes imipenem resistance in Pseudomonas aeruginosa. It was found that this mechanism could function only when the chromosomal beta-lactamase was expressed. Mutants lacking both the beta-lactamase and the D2 porin were almost as susceptible as those that lacked the beta-lactamase but retained the porin. Thus, imipenem resistance reflected an interplay of the enzyme and impermeability, not either factor alone. These findings suggest that the activity of a carbapenem more beta-lactamase stable than imipenem should be less affected by the porin loss. Meropenem approached this behavior.Keywords
This publication has 15 references indexed in Scilit:
- Susceptibility to -lactam antibiotics of mutant strains of Xanthomonas maltophilia with high- and low-level constitutive expression of L1 and L2 -lactamasesJournal of Medical Microbiology, 1991
- Imipenem- and meropenem-resistant mutants of Enterobacter cloacae and Proteus rettgeri lack porinsAntimicrobial Agents and Chemotherapy, 1991
- Invalidity for Pseudomonas aeruginosa of an accepted model of bacterial permeability to beta-lactam antibioticsAntimicrobial Agents and Chemotherapy, 1991
- Outer membrane protein D2 catalyzes facilitated diffusion of carbapenems and penems through the outer membrane of Pseudomonas aeruginosaAntimicrobial Agents and Chemotherapy, 1990
- Classification of beta-lactamases: groups 2c, 2d, 2e, 3, and 4Antimicrobial Agents and Chemotherapy, 1989
- Sensitivity of Escherichia coli to various β‐lactams is determined by the interplay of outer membrane permeability and degradation by periplasmic β‐lactamases: a quantitative predictive treatmentMolecular Microbiology, 1987
- The effect of imipenem on strains of Enterobacteriaceae expressing Richmond & Sykes Class I β-lactamasesJournal of Antimicrobial Chemotherapy, 1987
- Imipenem resistance in Pseudomonas aeruginosa resulting from diminished expression of an outer membrane proteinAntimicrobial Agents and Chemotherapy, 1987
- Emergence of Resistance to Imipenem During Therapy for Pseudomonas aeruginosa InfectionsThe Journal of Infectious Diseases, 1986
- Inducible Type I βlactamases of Gram-negative bacteria and resistance to βlactam antibioticsJournal of Antimicrobial Chemotherapy, 1986